Why is Cyanmethemoglobin method preferred for hemoglobin determination?

The haemoglobin is estimated with the help of cyanmethemoglobin curve. The advantages of this method are i) error due to subjective visual matching is avoided as spectrophotometer is used and hence reading is precise and reliable, ii) measures all forms of haemoglobin except sulphaemoglobin.

What method is used for hemoglobin estimation?

Direct cyanmethemoglobin method has been the gold standard for hemoglobin estimation but other methods like hemoglobin color scale, Sahli technique, Lovibond-Drabkin technique, Tallqvist technique, copper-sulfate method, HemoCue and automated haematology analyzers are also available.

What is Cyanmethemoglobin used for?

In the complete blood count, the erythrocyte count (RBC) was determined in a Neubauer chamber, while the hematocrit (Ht) was determined through the microhematocrit method and the hemoglobin concentration (Hb) through the cyanmethemoglobin method. The cyanmethemoglobin method was used to measure Hb.

What is Cyanmethemoglobin reagent?

Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components. Potassium ferricyanide oxidizes haemoglobin to methemoglobin and then to cyanmethemoglobin. The cyanmethemoglobin levels is estimated at 530nm.

What does Cyanmethemoglobin mean?

[ sī′ăn-mĕt′hē′mə-glō′bĭn, sī′ən- ] n. A relatively nontoxic compound of cyanide with methemoglobin, formed when methylene blue is administered in cases of cyanide poisoning.

Which instrument is used in oxyhemoglobin method and Cyanmethemoglobin method of Hb estimation?

Hemoglobinometers based on the cyanmethemoglobin/oxyhemoglobin method are available. The instrument has a built in filter and a scale and gives direct reading of Hb in g/dl. They are used in field surveys.

What is haemoglobin estimation?

This test measures the amount of haemoglobin (a protein found in red blood cells) in your blood and is a good indication of your blood’s ability to carry oxygen throughout your body. Haemoglobin carries oxygen to cells from the lungs.

What is hemoglobin estimation?

Overview. A hemoglobin test measures the amount of hemoglobin in your blood. Hemoglobin is a protein in your red blood cells that carries oxygen to your body’s organs and tissues and transports carbon dioxide from your organs and tissues back to your lungs.

What is Drabkin’s reagent?