How does CE MS work?

A CE/MS system works by first separating the ionic components of a sample by applying voltage to the sample. The ions will move through the capillary at different rates due to charge and frictional forces. The separated sample is then sprayed into the mass spectrometer which produces a spectra.

What is CE SDS analysis?

Abstract. Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) is an analytical method to assess the purity of proteins, commonly applied to monoclonal antibodies (mAbs) in the biopharmaceutical industry.

How do you test for Monoclonal Antibodies?

Once monoclonal antibodies for a given substance have been produced, they can be used to detect the presence of this substance. Proteins can be detected using the Western blot and immuno dot blot tests.

What is monoclonal antibody characterization?

mAb Structural Characterisation Mass spectrometry is a powerful technique for confirming the primary sequence with intact mass analysis and peptide mapping. This allows expected sequences to be confirmed and terminal amino acid homogeneity to be assessed.

What is capillary zone electrophoresis?

Capillary zone electrophoresis (CZE) is a technique successfully used for the separation of proteins, peptides, and nucleic acids. Other applications where CZE may be useful include analysis of inorganic anions and cations, such as those typically separated by ion chromatography.

Is CE SDS quantitative?

Capillary electrophoresis sodium dodecyl sulfate (CE-SDS) is the only way to obtain automated, quantitative purity data (i.e. glycosylated vs non-glycosylated) on both intact and reduced monoclonal antibodies. It is quantitative, automated and fast.

What is reduced CE SDS?

Capillary electrophoresis sodium dodecyl sulfate (CE-SDS), either in reduced (rCE-SDS) or non-reduced (nrCE-SDS) form, is widely used for purity evaluation and impurity analysis of monoclonal antibody (mAb) drugs.

How are monoclonal antibodies produced identified and screened?

A typical monoclonal antibody production process Immunization of mice & isolation of splenocytes – Mice are immunized with an antigen and later their blood is screened for antibody production. The antibody-producing splenocytes are then isolated for in vitro hybridoma production.

Which analysis is performed in antibody characterization?

A method used extensively for the identification and characterization of antibodies is agar diffusion. The method was originally devised by Ouchterlony. The procedure is readily performed on a microscope slide.

What is Antibody characterization?

Antibody characterization is important in research and biopharmaceutical development. All therapeutic proteins produced from current technologies have heterogeneities in the final product because various modifications occur during different stages of production.