What materials are needed for micropropagation?

Materials

  • 1 vial of Murashige Skoog (MS) medium.
  • 1 L sterile distilled water.
  • 8 g of agar/L.
  • 30 g sucrose/L.
  • 1.5-L or 2-L container in which to prepare the growth medium.
  • Small amounts of 1M NaOH and 1M HCl to adjust the pH of the medium.
  • 30 flat-bottom culture tubes with closures.
  • Glass aquarium or box lined with plastic.

What equipment is used in tissue culture?

Autoclave: Autoclave is used to sterilize medium, glassware and tools for the purpose of plant tissue culture. The same equipment is used in hospitals to sterilize gauge, cotton, tools and linen, etc.

What plant tissue is used in micropropagation?

Micropropagation starts with the selection of plant tissues (explant) from a healthy, vigorous mother plant [15]. Any part of the plant (leaf, apical meristem, bud and root) can be used as explant. The whole process can be summarized into the following stages as shown in Figure 2.

What are the ingredients for tissue culture?

The essential elements in plant cell or tissue culture media include, besides C, H and O, macroelements: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulphur (S) for satisfactory growth and morphogenesis.

What equipment is used to grow cells?

Cell Culture Incubators Cell cultures require a strictly controlled environment in which to grow. Cell culture incubators are used routinely to provide the correct growth conditions, such as temperature, degree of humidity and CO2 levels, in a controlled and stable manner.

What is needed for a tissue culture lab?

The tissue culture laboratory must have four basic environments: an office, a washing and media preparation room, a transference room, and a culture room, which require a minor to major grade of cleaning, as well as a minor to major restriction on personnel access.

What are the steps involved in micropropagation?

The process of micropropagation can be divided into four stages:

  • Initiation stage. A piece of plant tissue (called an explant) is (a) cut from the plant, (b) disinfested (removal of surface contaminants), and (c) placed on a medium.
  • Multiplication stage.
  • Rooting or preplant stage.
  • Acclimatization.

How do you make agar for tissue culture?

How to Prepare Agar for Plant Tissue Culture

  1. Take a beaker and add 800 ml water to it.
  2. Add 4g of MS media, 30 grams of sugar, 10 grams of agar, and 2 ml of PPM (Plant Preservative Mixture-to avoid any kind of contamination from your cultures).