What is Oligo dT?

Description. Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.

What is Oligo dT primer used for?

Oligo(dT)15 Primer is suitable for use as a primer for first-strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly(A) tail of mRNA.

What does Oligo dT bind to?

Oligo(dT) primers are designed so that they bind to the complementary poly(A) tails of messenger RNA (mRNA).

Why do we use Oligo dT?

Oligo(dT) primers are a favorite choice for two-step cDNA synthesis reactions because of their specificity for mRNA and because they allow many different targets to be studied from the same cDNA pool.

How do oligo dT beads work?

The use of Dynabeads® Oligo (dT)25 relies on base pairing between the poly A tail of messenger RNA and the oligo dT sequences bound to the surface of the beads. After annealing, the vial is placed on a magnet (Dynal® MPC™) to concentrate the beads with their bound mRNA at the side of the tube.

What is a DNA oligo?

Oligonucleotides, or oligos, are short single strands of synthetic DNA or RNA that serve as the starting point for many molecular biology and synthetic biology applications! From genetic testing to forensic research and next-generation sequencing, an oligo may very well be the starting point.

What is oligo dT cellulose?

Oligo(dT) Cellulose is an affinity matrix designed for the isolation of poly(A)+ RNA (mRNA) (1). The matrix is synthesized by a modification of the procedure of Gilham (2). Chains of deoxythymidylate up to 20 nucleotides long are covalently attached to a cellulose matrix via the terminal 5´-phosphate group.

What is cDNA library used for?

cDNA libraries have been broadly used to determine the expressed portion of protein-coding genes in eukaryotes. The construction of a cDNA library involves the extraction and purification of mRNA (Fig. 2.8).

What are oligo used for?

What is oligo mixture?

Standard “wobbles” or “mixed bases” are mixtures of two or more different bases at a given position within the sequence. Wobbles are often incorporated into oligonucleotide probes designed to hybridize to an unknown gene that encodes a known amino acid sequence.