What is goat serum used for?

What is goat serum used for?

Background. Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk.

How do you reconstitute normal goat serum?

Restore with distilled water: 10.0 mL (600 mg package size) and 2.0 mL (120 mg package size). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.

Why do we use goat serum for blocking?

The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

How long does goat serum last in fridge?

The 20 ml provided therefore is all goat serum. For most IHC and IF tissue staining applications, an aliquot is taken and diluted in assay buffer such as PBS or TBS to 2-10% (v/v). This working solution can be stored in the fridge and used for up to one week.

What is donkey serum?

Donkey Serum is a sterile filtered preparation of donkey serum prepared from donkey whole blood following clotting.

How does BSA blocking work?

BSA blocking is a routine practice among clinicians and researchers working on immunoassays throughout the world. The primary role of BSA is to prevent the non-specific binding by blocking the leftover spaces over solid surface after immobilization of a capture biomolecule.

How do you make a blocking buffer for immunofluorescence?

Blocking Buffer: Purchase ready-to-use Immunofluorescence Blocking Buffer (#12411), or prepare a 1X PBS / 5% normal serum / 0.3% Triton™ X-100 buffer by adding 0.5 mL normal serum from the same species as the secondary antibody (e.g., Normal Goat Serum (#5425) and 30 µL Triton™ X-100 to 9.5 mL 1X PBS. Store at 4°C.

What is donkey serum used for?

General notes. Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

How does serum blocking work?

Blocking with sera or a protein blocking reagent prevents non-specific binding of antibodies to tissue or to Fc receptors. Theoretically, any protein that does not bind to the target antigen can be used for blocking.

Can you freeze serum?

Multiple thaw/freeze cycles should be avoided as they will hasten the degradation of serum nutrients and can induce the formation of insoluble precipitates. For this reason, serum should never be stored in “frost-free” freezers.

How do you thaw normal goat serum?

Place each bottle in 37° water bath. Gently swirl or shake the bottles every 10 minutes until the serum is completely thawed, or use a shaking water bath to agitate the serum while thawing.

What is normal donkey serum used for?