What does CREB bind to?
What does CREB bind to?
cAMP response elements
CREB-TF (CREB, cAMP response element-binding protein) is a cellular transcription factor. It binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the genes.
Does cAMP bind to CREB?
The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin …
Where can I find promoter binding sites?
Go to Eukaryotic Promoter database, put in the NCBI accession number for your gene of interest and then search for the transcription factors of your interest for that promoter sequence. Promoter sequence can also be limited (eg. -500 to +100) or extended (-1000 to +1) the way you like.
What is binding site of promoter?
A promoter is a sequence that initiates and regulates the transcription of a gene. Protein binding sites in a promoter represent the most crucial elements and the corresponding proteins are called transcription factors (TFs).
Is CREB a transcription activator?
CREB is a transcription factor that regulates diverse cellular responses, including proliferation, survival, and differentiation. CREB is induced by a variety of growth factors and inflammatory signals and subsequently mediates the transcription of genes containing a cAMP-responsive element.
How does PKA activate CREB?
Transcriptional regulation via adenylate cyclase signalling pathways is mediated by a family of cyclic AMP responsive nuclear factors, including CREB. The activation function of CRE binding proteins is modulated by phosphorylation by several kinases (for example, PKA) and other cellular cofactors.
How do you predict transcription factor binding sites?
Prediction of transcription factor binding sites is generally performed by scanning a DNA sequence of interest with a position weight matrix (PWM) for a transcription factor of interest [6, 7] and various pattern-matching tools have been developed for this purpose.
What is consensus DNA binding site?
A protein binding site, represented by a consensus sequence, may be a short sequence of nucleotides which is found several times in the genome and is thought to play the same role in its different locations.
How do you identify transcription factor binding sites?
