How does the Wagner reagent work for detecting alkaloids?

In Wagner reagent preparation, iodine reacts with I- ion from potassium iodide producing I3- ion (brownish solution). In the Wagner test, the metal ion of K+ will bind as covalent coordinate bonding with nitrogen to alkaloid producing a complex precipitate of potassium-alkaloid. The reaction is predicted as Figure 2.

What is Wagner’s reagent used for?

An aqueous solution of iodine and potassium iodide; used for microchemical analysis of alkaloids.

What reagent is used to test for alkaloids?

Dragendorff’s reagent is a color reagent to detect alkaloids in a test sample or as a stain for chromatography plates.

How do you test for the presence of alkaloids?

Tests for alkaloids By adding 1 mL of Dragendorff”s reagent to 2 mL of extract, an orange red precipitate was formed, indicating the presence of alkaloids. (b) Mayer’s test. Few drops of Mayer’s reagent were added to 1 mL of extract. A yellowish or white precipitate was formed, indicating the presence of alkaloids.

What is the composition of Wagner’s reagent?

Wagner’s Reagent: 2.5 gm iodine is dissolve in 12.5 gm of potassium iodide (KI 2 ); add 250 ml of water to produce solution.

Why are Alkaloidal reagents called?

The name alkaloid (“alkali-like”) was originally applied to the substances because, like the inorganic alkalis, they react with acids to form salts.

How does the Dragendorff reagent work for detecting alkaloids?

Dragondorff’s reagent (potassium iodide-bismuth nitrate) is widely used reagent to detect alkaloids. The heavy metal atom (BiI4) combines with the nitrogen in the alkaloid to form ion pairs. This ion pair forms insoluble orange red colored complex.

What are alkaloid drugs?

Alkaloid: A member of a large group of chemicals that are made by plants and have nitrogen in them. Many alkaloids possess potent pharmacologic effects. The alkaloids include cocaine, nicotine, strychnine, caffeine, morphine, pilocarpine, atropine, methamphetamine, mescaline, ephedrine, and tryptamine.

What is Dragendorff reagent?

DR is a solution of potassium bismuth iodide composing of basic bismuth nitrate (Bi(NO₃)₃), tartaric acid, and potassium iodide (KI), and when contact with alkaloids DR produces an orange or orange red precipitate.

Which of the following are alkaloids?

Some common alkaloids are morphine, strychnine, quinine, ephedrine and nicotine. Alkaloids are found primarily in the plants and are especially common in the certain families of the flowering plants.

How are alkaloids classified?

Often, alkaloids are classified based on their chemical structure. For example, alkaloids that contain a ring system, which is called indole, are referred to as indole alkaloids. Based on the principal classes of alkaloids are the tropanes, pyrrolidines, indoles, isoquinolines, and steroids and terpenoids.