How does Illumina HiSeq 2500 work?

How does Illumina HiSeq 2500 work?

The HiSeq 2500 System harnesses proven Illumina SBStechnology to deliver highly accurate data and robust performance for a broad range of applications. SBS uses a reversible-terminator method, with fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands.

What is the difference between NovaSeq and HiSeq?

NovaSeq 6000 The Illumina NovaSeq provides a massive upgrade in sequencing throughput compared to the HiSeq 4000. There are more stringent library requirements and requires a larger sample size.

What HiSeq 2500?

The HiSeq 2500 System is a powerful sequencing system with the flexibility to perform multiple applications. High-quality data using proven Illumina SBS chemistry has made it the instrument of choice for major genome centers and research institutions throughout the world.

What is HiSeq used for?

With a unique mix of high output, long reads, and paired-end sequencing, HiSeq is a powerful tool for de novo sequencing of species without a reference genome.

What is MiSeq used for?

The MiSeq is an integrated instrument that performs clonal amplification, sequencing, and data analysis (base calling, alignment, variant calling, and reporting) in a single run.

What is HiSeq sequencing?

How many reads on a NovaSeq?

NovaSeq performs whole-genome sequencing more efficiently and cost-effectively than ever. Its scalable output generates up to 6 Tb and 20 billion reads in dual flow cell mode with simple streamlined automated workflows.

How many reads HiSeq?

The HiSeq 4000 generally gives 280-330 million reads per lane for a flowcell total of around 2.2 billion reads. While the patterned flow cell used on the 4000 is fixed at 480 million wells/reads per lane, Illumina recommends between 60-70% passed filter (PF) to maximize the number of unique reads in the lane.