How do you make bromocresol green solution?
How do you make bromocresol green solution?
Bromocresol Green Indicator Solution: Dissolve 50 mg of bromocresol green in 0.72 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). After the solution is effected, add sufficient water to produce 100 ml.
What is bromocresol green solution?
Bromocresol green (BCG) is a triphenylmethane dye that is used as a pH indicator for titration, DNA agarose gel electrophoresis, and microbiological growth media. Its chemical formula is C21H14Br4O5S. The aqueous indicator is yellow below pH 3.8 and blue above pH 5.4.
In which solution will bromocresol green appear yellow?
1 Answer. In aqueous solution, bromocresol green will ionize to give the monoanionic form (yellow), that further deprotonates at higher pH to give the dianionic form (blue), which is stabilized by resonance: The acid dissociation constant (pKa) of this reaction is 4.8.
What color would a bromocresol green solution be at pH 2?
yellow color
In this question, the pH value is 2.0, which is less than 3.8, therefore bromcresol green will be of yellow color.
What pH does bromocresol green change color?
Bromo Cresol Green Indicator has wide range of application so follow apropriate direction as per application protocol. Green coloured solution. Clear without any particles. At pH 3.8, the indicator turns yellow and at pH 5.4, the indicator is blue-green.
Is BCG a pH indicator?
Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). It belongs to a class of dyes called sulfonephthaleins. It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations.
What color is bromocresol green in basic solutions?
blue
Bromocresol green (BCG) (3′, 3”, 5′, 5”-tetrabromo-m-cresolsulfonphthalein) (Fig. 1b) is a sulfonphthalein dye, with a transition range of pH 3.8 to 5.4. In the acidic form, it appears yellow, and in the basic form, it is blue. It is used as a pH indicator and as a tracking dye for DNA agarose gel electrophoresis.
What color bromocresol green when it is put into a basic solution?
blue-green color
The acid dissociation constant (pKa) of this reaction is 4.8. Tap water is sufficiently basic to give a solution of bromocresol green its characteristic blue-green color.
What color is bromocresol green indicator in basic solutions?
What color does bromocresol green turn?
yellow
| Indicator | Low pH color | Transition pH range |
|---|---|---|
| Congo red | blue-violet | 3.0–5.0 |
| Methyl orange | red | 3.1–4.4 |
| Bromocresol green | yellow | 3.8–5.4 |
| Methyl red | red | 4.4–6.2 |
How do you use bromocresol green indicator?
Bromocresol green (BCG) is a dye of the triphenylmethane family (triarylmethane dyes). It belongs to a class of dyes called sulfonephthaleins. It is used as a pH indicator in applications such as growth mediums for microorganisms and titrations….Bromocresol green.
| Bromocresol green (pH indicator) | ||
|---|---|---|
| below pH 3.8 | above pH 5.4 | |
| 3.8 | ⇌ | 5.4 |
Which solution has the highest pH?
Explanation: 50.0 mL 0.0500 M calcium hydroxide, Ca(OH)2 (aq) has the highest pH value. Sodium hydroxide and Calcium hydroxide are strong bases so they completely ionize in solution.
