Do monocytes express CD33?

For example, mature monocytes are typically characterized by the expression of CD33, CD11b, CD14, HLA-DR, and CD16 whereas granulocytes are characterized by CD33, CD11b, CD15, and CD66b.

What is CD33 a marker for?

CD33 is an excellent myeloid marker and is commonly used for the diagnosis of AML. However, approximately 10–20% of B-lymphoblastic or T-lymphoblastic leukemia/lymphomas may aberrantly express CD33.

What cells are CD33 positive?

CD33 was detected on blasts of 85–90% of patients presenting with AML as well as on normal myeloid progenitors and myelocytes. Interestingly, CD33 seems to be restricted to hematopoietic cells,12, 13 but absent on normal hematopoietic stem cells,14, 15, 16, 17 making it an ideal target for AML therapy.

Do monocytes express CD117?

Mature monocytes are positive for CD11b, CD11c, CD13, CD14, CD33, and CD64, and may express CD2 and CD4. Blasts in acute myeloid leukemias (AML) with minimal differentiation have low SSC and moderate CD45 expression and are positive for CD34, CD117, CD13, HLA-DR, and CD33 and may be positive for TdT, CD4, and CD11c.

What markers do monocytes express?

Monocytes express typical blood monocyte markers, such as CD14, CD11b, CCR2, and CD16, but at extravascular sites they possess higher levels of CD141, CD11c, HLA-DR, and CCR7, indicating a tissue-imprinted phenotypic change that is reminiscent of DCs (111, 114).

Do human monocytes express CD11c?

CD11c is a complement receptor which is often exploited as a single marker to track murine dendritic cells, whereas all human monocytes express this molecular marker and may retain protein expression following tissue extravasation.

What is CD33 positive AML?

In AML, one of the antigens usually expressed is CD33. Physiologically, CD33 expression is restricted to early multilineage hematopoietic progenitors, myelomonocytic precursors and more mature myeloid cells, being absent on normal pluripotent hematopoietic stem cells. About 85–90% of AML cases express the CD33 antigen.

What does CD33 stand for?

CD33 or Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67) is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells.

Do monocytes express CD115?

In spleen, lineage− (Ly6G, NK1. 1, CD3, and CD19) CD11b+ CD11c− splenocytes were identified as monocytes based on their expression of CD115, Ly6C/Gr1, and F4/80 (Fig. 5 a) (32). We observed that these spleen CD115+ Gr1+ monocytes produced TNF-α, iNOS, and ROI in mice during infection with Lm (Fig.

Do monocytes express CD19?

Expression of CD19, as demonstrated by B4, was always associated with the population expressing monocyte markers.

Do monocytes express MHC-II?

MHC-II is constitutively expressed by immune cells including B cells, monocytes, macrophages, and dendritic cells (DCs) and even non-hematopoietic cells can express MHC-II under inflammatory conditions.

Do all monocytes express CD11c?