Can apoptosis occur without caspase-3?

This is possible, you can still get apoptosis without these proteins being upregulated. For example, in Caspase independent apoptosis, caspases are not cleaved.

How does caspase-3 and 7 cause apoptosis?

Caspase 3 controls DNA fragmentation and morphologic changes of apoptosis, whereas caspase 7 plays little role in these processes. In contrast, caspase 7 appears to be more important to the loss of cellular viability, although the combined role of both caspases is crucial in this area.

What is the role of caspase-3 in apoptosis?

Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular structures such as DNA fragmentation or degradation of cytoskeletal proteins (1).

What is MCF-7 cancer cell line?

4.8. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].

What does caspase-3 indicate?

Thu, 04/07/2016 – 13:44. Caspases, or cysteine-dependent aspartate specific proteases, are a family of enzymes crucial for initiating and executing apoptosis within a cell, an important biological event especially during organ development (1).

What does caspase-7 do in apoptosis?

However both of these effector caspases have distinct functions during apoptosis. Recent studies have indicated caspase-3 is needed for efficient cell killing and can also block ROS production (3). Caspase-7, on the other hand, is responsible for ROS production and aids in cell detachment during apoptosis (3).

Does MCF-7 have HER2?

Two particularly important members of this large family are the epidermal growth factor receptor (EGFR), which is activated by the epidermal growth factor (EGF), and the human epidermal growth factor receptor-2 (HER2), both present in MCF-7 cells (7).

Is MCF-7 triple negative?

MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.

What is caspase-3 7?

Description. The Caspase-Glo® 3/7 Assay(a,b) is a homogeneous, luminescent assay that measures caspase-3 and -7 activities. The assay provides a luminogenic caspase-3/7 substrate, which contains the tetrapeptide sequence DEVD, in a reagent optimized for caspase activity, luciferase activity and cell lysis.

Do MCF-7 cells overexpress HER2?

In most of the MCF7 subclones that contain high EGFR overexpressing cells, the cells with EGFR levels exceeding 80 000 molecules per cell exhibit only moderately elevated levels of HER2 (mostly in the range of 200 000–600 000 molecules per cell).