How does Antarctic Phosphatase work?

Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs).

What is NEB Buffer?

Product Listing Product Overview. For greater flexibility, NEB provides a selection of buffers for optimal enzyme activity, as well as for use with its protein expression and purification, cloning and RNA products. These buffers are available separately, or in bulk volumes, upon request.

What is the role of CutSmart buffer?

Our CutSmart Buffer incorporates BSA to enable even more enzymes to cut in a single buffer (>200 enzymes). This allows for enhanced ease of use especially when doing double digests. In addition, it eliminates the extra tube of BSA and means one less thing to think about when setting up restriction enzyme digests.

Which is the substrate for phosphatase enzyme?

Based on the optimum pH for the activity, phosphatases are of two kinds: acid and alkaline. Acid phosphatases show maximum activity at acidic pH around 6 whereas alkaline phosphatases show maximum activity at alkaline pH around 11. The p-nitrophenyl phosphate acts as a substrate for both acid and alkaline phosphatases.

What type of reaction is catalyzed by phosphatase?

Phosphatases catalyze the hydrolysis of a phosphomonoester, removing a phosphate moiety from the substrate.

What is CutSmart buffer made of?

Article Snippet: The CutSmart® buffer ( New England Biolabs® ) contains 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/mL BSA.

What is the difference between rCutSmart and CutSmart?

FAQ: What is the difference between CutSmart Buffer and rCutSmart Buffer? rCutSmart Buffer contains Recombinant Albumin (NEB #B9200) while CutSmart Buffer contains BSA (NEB #B9000).

What is the substrate of protein phosphatase?

Three distinct amino acids are substrates for protein phosphorylation and dephosphorylation. Protein phosphatases are structurally and functionally diverse enzymes, which are subdivided by their substrate specificity into two distinct families: tyrosine-specific and serine/threonine-specific phosphatases.