What is kligler iron agar used for?

Kligler Iron Agar is used for the differentiation of microorganisms in the Enterobacteriaceae family on the basis of dextrose and lactose fermentation and hydrogen sulfide production.

How is kligler iron agar different from TSI?

TSI is similar to Kligler’s iron agar (KIA), except that Kligler’s iron agar contains only two carbohydrates: glucose (0.1%) and lactose (1%). 0.1% glucose: If only glucose is fermented, only enough acid is produced to turn the butt yellow.

How do you inoculate kligler iron agar?

Using an inoculating needle, inoculate KIA with a light inoculum from a pure, 18-24 hour culture growing on solid medium. Streak the slant back and forth across the entire surface and stab to the bottom of the agar butt. 3. Incubate tube in ambient air at 33-37°C for 18-24 hours and with cap loosened.

What is the principle of TSI?

Principle of TSI test: The presence of fetrous sulphate and sodium thiosulphate fulfills the demand of sulphur and there is the indicator phenol red for the indication of change in the invironment of the media due to the production of acid or alkali.

What is kligler iron agar test?

What is Kligler Iron Agar Test? Kligler iron agar test is used for detecting the fermentation of carbohydrate in the medium. Here the reaction of the KIA helps us to include or exclude a particular bacterial species to isolate it from the family of Enterobacteriaceae.

How does the kligler iron slant measure lactose fermentation?

Lactose fermenters produce yellow slants and butts because of lactose fermentation. The high amount of acids thus produced helps to maintain an acidic pH under aerobic conditions. Tubes showing original colour of the medium indicates the fermentation of neither glucose (dextrose) nor lactose.

How do you do a kligler iron agar test?

Method

  1. With an inoculating needle, pick the center of well-isolated colonies obtained from solid culture media.
  2. Stab the center of the medium into the deep of the tube to within 3-5mm from the bottom.
  3. Withdraw the inoculating needle and streak the surface of the slant.
  4. Loosen closure on the tube before incubating.

Why is TSI test used?

The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism’s ability to ferment sugars and to produce hydrogen sulfide. It is often used to differentiate enteric bacteria including Salmonella and Shigella.

What is the pH indicator in TSI Agar?

Phenol red
Triple-sugar iron agar (TSI agar) is another example of a multi-test agar. It tests for the fermentation, with or without gas production, of glucose, lactose, and sucrose. It also tests for the production of hydrogen sulfide from amino acids. Phenol red is the pH indicator used in this test medium.

What is the difference between KIA and TSI?

The formulae for TSI agar and KIA are identical except that in addition to glucose and lactose, TSI agar contains sucrose. TSI forms hydrogen sulfide (H2S) and is used to determine whether a gram-negative rod ferments glucose and lactose or sucrose.

What is TSI positive?

How do I read my TSI test results?

Expected Results

  1. An alkaline/acid (red slant/yellow butt) reaction: It is indicative of dextrose fermentation only.
  2. An acid/acid (yellow slant/yellow butt) reaction: It indicates the fermentation of dextrose, lactose and/or sucrose.