What is the SAOS 2 cell line?

Saos-2 is a human osteosarcoma cell line, which displays several osteoblastic features. These cells express receptors for 1,25-dihydroxyvitamin D3 and have high basal alkaline-phosphatase activity. They express the parathyroid hormone (PTH) receptor and produce cyclic AMP in response to treatment with PTH.

What is human osteosarcoma cell lines?

Background: Osteosarcomas are malignant bone tumors consisting of cells with abnormal cellular functions. Although osteosarcoma-derived cells are commonly used for osteoblastic models, the molecular composition of the osteosarcoma extracellular matrix (ECM) is not well characterized.

What are MC3T3 E1 cells?

The osteoblastic cell line MC3T3-E1 has been established from a C57BL/6 mouse calvaria and selected on the basis of high alkaline phosphatase (ALP) activity in the resting state. Cells have the capacity to differentiate into osteoblasts and osteocytes and have been demonstrated to form calcified bone tissue in vitro.

Why use U2OS cells?

U2OS cells are much more adherent than HEK 293 and 293T cells. As such these are better suited for immunofluorescence studies when compared to HEK which loosely attach to coated cover slips.

Why are U2OS cells used?

The U2OS cell line is extensively used as a circadian rhythm model, including in high-throughput screening studies (Zhang et al., 2009).

What are 3T3 L1 cells?

3T3-L1 is a fibroblast that was isolated from the embryo of a mouse. This cell line can be used to study the basic cellular mechanisms associated with diabetes, obesity, and related disorders.

How big are U2OS cells?

We determined the nuclear dimensions of freely growing U2OS cells to be 16 ± 2 μm in width, 23 ± 3 μm in length, and 4 ± 1 μm in height (n = 60).

Are U2OS cells adherent?

All Answers (5) Suspended U2OS cells remain viable and they will re-adhere and keep growing but their growth is likely to be affected while they remain in suspension. The cell cycle length of U2OS cells is ~18–20 h, and so a 48-h time course would be expected to have only ~2.5 cell cycles.

What are calu 1 cells?

Description. Calu-1 is a non-small-cell lung cancer (NSCLC) cell line that grows in adherent culture and displays epithelial morphology. These cells express wildtype LKB1, wildtype EGFR, and mutant K-Ras (G12C).