What type of promoter is CMV?

The CMV promoter is a commonly used promoter for the production of high level recombinant protein in mammalian cells17. However, the expression level of the transgene driven by CMV promoter decreases with extended culture times because of transcriptional silencing, which is associated with DNA methylation18, 19.

Is CMV a promoter?

The cytomegalovirus (CMV) immediate early promoter has been extensively developed and exploited for transgene expression in vitro and in vivo, including human clinical trials. The CMV promoter has long been considered a stable, constitutive, and ubiquitous promoter for transgene expression.

What activates CMV promoter?

Dimethylsulfoxide and 5-Aza-2′-deoxycytidine can activate the cytomegalovirus promoter in both cell types by overlapping mechanisms.

What is the CB promoter?

The cytomegalovirus (CMV) early enhancer/promoter and the hybrid CMV enhancer/chicken β-actin (CBA) promoter are commonly used in gene transfer studies with both therapeutic and reporter genes. These promoters are typically used to provide robust, long-term expression in all cell types.

Does CMV promoter work in T cells?

The CAG and CMV promoter-containing vectors were not particularly effective in driving GFP expression in human T cells. Because of the generally poor GFP expression from the CMV-promoted vector, it was not used in subsequent experiments.

What is CMV enhancer?

The cytomegalovirus (CMV) major immediate early (MIE) enhancer-containing promoter regulates the expression of the downstream MIE genes, which have critical roles in reactivation from latency and acute infection. The enhancer consists of binding sites for cellular transcription factors that are repeated multiple times.

What is the sequence of the T7 a1 promoter?

The T7A1 promoter’s wild-type (wt) sequence is characterized by the presence of an AT-rich UP element extending to –71, a near consensus –35 sequence (TTGACT instead of TTGACA) and a non-consensus –10 sequence (GATACT instead of TATAAT).

What is a U6 promoter?

U6 is a type III RNA polymerase III promoter commonly used for driving small hairpin RNA (shRNA) expression in vector-based RNAi. In the design and construction of viral vectors, multiple transcription units may be arranged in close proximity in a space-limited vector.