What is the Folin Wu method?

Principle. Glucose reduces the cupric ions present in the alkaline copper reagent to cuprous ions or the cupric sulfate is converted into cuprous oxide, which reduces the phosphomolybdic acid to phosphomolybdous acid, which is blue when optical density is measured at 420 nm.

What is Folin Wu filtrate?

The tungstic acid method introduced by Folin and Wu (1) has proven by far the most desirable one for obtaining blood filtrates free from protein. Folin and Wu direct that 1 volume of blood be first diluted with 7 volumes of distilled water.

What are the method of glucose estimation?

There are three basic approaches to the laboratory measurement of blood glucose concentration: reducing methods, condensation methods, and enzymatic methods.

What is the principle of glucose estimation?

The enzyme glucose oxidase reacts with glucose, water, and oxygen to form gluconic acid and hydrogen peroxide. The hydrogen peroxide can then be used to oxidize a chromogen or the consumption of oxygen measured to estimate the amount of glucose present.

What is Somogyi Nelson method?

The nelson-somogyi method is used to measure reducing sugar by using copper reagents and arsenolmolibdat. The working principle of somogyi-nelson is the amount of deposro oxide deposits that react with arsenomolibdate which is reduced to molybdine blue and the blue color is measured absorbance.

What is glucose oxidase method?

In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen peroxide. The hydrogen peroxide is then oxidatively coupled with a chromogen to produce a colored compound which may be measured spectroscopically.

What is Folin Wu tube?

Folin Wu Tubes were early instruments used to measure glucose.

Which is a better method Folin Wu or Somogyi Nelson?

The new technique which has been compared to glucose oxidase method, Somogyi’s procedure and Folin-Wu method is easier and more accurate than the Somogyi’s original method, and gives blood glucose values almost identical with that obtained with glucose oxidase procedure.

Which is a better method Folin-Wu or Somogyi Nelson?

What is DNS method?

The dinitrosalicylic acid (DNS) method gives a rapid and simple estimation of the extent of saccharification by measuring the total amount of reducing sugars in the hydrolysate.

Why is glucose oxidase used?

Glucose oxidase enzymes are used to remove small amounts of oxygen from food products or glucose from diabetic drinks. Glucose oxidase is playing an important role in color development, flavor, texture, and increasing the shelf life of food products (Khurshid et al., 2011).

What is the purpose of the glucose oxidase test?

Glucose oxidase is widely used for the determination of free glucose in body fluids (medical testing), in vegetal raw material, and in the food industry. It also has many applications in biotechnologies, typically enzyme assays for biochemistry including biosensors in nanotechnologies.