What is bold basal medium?

Bold’s Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965).

How do you use Bold’s basal medium?

Directions

  1. To approximately 900 mL of dH2O add each of the components in the order specified into a 1-Liter graduated cylinder.
  2. Add one drop of each of the following solutions to the cylinder.
  3. Bring the total volume to 1L with dH2O.
  4. Transfer to a 2-Liter Erlenmeyer flask.
  5. Autoclave to sterilize; allow to cool.

What are the main components in the basal medium?

13.3. Basal media contain amino acids, glucose, and ions (calcium, magnesium, potassium, sodium, and phosphate) essential for cell survival and growth.

How do you get a basal medium?

Dissolve 5 mg riboflavin into the bottle. Dissolve 5 mg thiamine into the bottle. Dissolve 5 mg nicotinic acid into the bottle. Dissolve 5 mg pantothenic acid into the bottle.

What is BG 11 medium?

BG11 Broth is a universal medium for the cultivation and maintenance of blue green algae (cyanobacteria).

What is Zarrouk medium?

Zarrouk’s medium has successfully served as the standard medium (SM) for Spirulina culture for many years (Zarrouk, 1966). Consequently, many media have been developed using seawater (Faucher et al., 1979), sewage water (Saxena et al., 1982) and industrial effluents (Tanticharoen et al., 1993).

Which media is used for algae?

Fertilizer for algae: Salt broth and agar plate are the two major types of growth media used for cell culture. Typically, cultures are grown in a liquid salt broth with corresponding nutrients and maintained under optimal conditions.

What is basal media in microbiology?

BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. Examples: Nutrient broth, nutrient agar and peptone water. Staphylococcus and Enterobacteriaceae grow in these media.

Does basal media serum?

DMEM is a basal medium and contains no proteins or growth promoting agents. Therefore, it requires supplementation to be a “complete” medium. It is most commonly supplemented with 5-10% Fetal Bovine Serum (FBS).

Why is BG-11 medium for cyanobacteria?

Gibco® BG-11 Media is optimized for the growth and maintenance of select Cyanobacteria including Synechococcus elongates. The formulation comes ready-to-use at 1X to let you avoid laborious media preparation steps.