What are the principle differences between a spectrophotometer and a Spectrofluorometer?

The key difference between spectrophotometer and spectrofluorometer is that spectrophotometer involves the measurement of absorption whereas spectrofluorometer involves the transition of polyatomic fluorescent molecules from their higher energy level to a ground state by lowering their energy level through emission of …

What is the principle of a spectrophotometer?

Spectrophotometer Principle. The spectrophotometer is an instrument which measures the amount of light that a sample absorbs. The spectrophotometer works by passing a light beam through a sample to measure the light intensity of a sample.

How does a Spectrofluorometer work?

Spectrofluorometers (or fluorescence spectrophotometers) measure the fluorescence signature of an analyte in a sample based on its specific excitation and emission wavelengths. The fluorescence signature can be correlated to the concentration level of the analyte in the sample.

What is the principle of beers law?

Beer’s Law states that the concentration of a chemical solution is directly proportional to its absorption of light. The premise is that a beam of light becomes weaker as it passes through a chemical solution. The attenuation of light occurs either as a result of distance through solution or increasing concentration.

What is difference between Spectrofluorometer and fluorometer?

The difference between them is the way they select the wavelengths of incident light; filter fluorometers use filters while spectrofluorometers use grating monochromators. Filter fluorometers are often purchased or built at a lower cost but are less sensitive and have less resolution than spectrofluorometers.

What is spectrophotometer and its function?

The spectrophotometer is an optical instrument for measuring the intensity of light relative to wavelength. Electromagnetic energy, collected from the sample, enters the device through the aperture (yellow line) and is separated into its component wavelengths by the holographic grating.

Which type of monochromator is used in spectrofluorometer?

Introduction The most compelling reason for using a double monochromator on a spectrofluorometer is to reduce the stray light level. Stray light usually refers to any radiation at wavelengths other than the selected wavelength, which may exit the monochromator.

Why is beer and Lambert Law important for spectrophotometer?

The Beer-Lambert law states that there is a linear relationship between the concentration and the absorbance of the solution, which enables the concentration of a solution to be calculated by measuring its absorbance.

What is the purpose of fluorometer?

A fluorometer (and fluorimeter) measures the fluorescence or light emitted by different fluorescing objects. Fluorescence occurs when light of specific wavelength hits and excites electrons in a sample, and the electrons in that sample instantly emit or fluoresce light of a different wavelength.