How many cells can a 6 well plate hold?
How many cells can a 6 well plate hold?
Useful information for various sizes of cell culture dishes and flasks
| Catalog No. | Cells at confluency* | |
|---|---|---|
| Dishes | ||
| 6-well | 140675 | 1.2 x 106 |
| 12-well | 150628 | 0.5 x 106 |
| 24-well | 142475 | 0.24 x 106 |
What is the function of a well plate?
Well plates are used in virology, serology, microbiology and a myriad of other life science and drug discovery laboratories. The microplate is a simple, relatively low-tech, cost-effective, humble and unassuming tool found in labs across the world.
What are tissue culture treated plates?
Tissue-culture treated plates The tissue culture treatment process involves exposing a polystyrene microplate to a plasma gas in order to modify the hydrophobic plastic surface to make it more hydrophilic.
How much volume does a 24-well plate have?
0.5-2.39 mL
CulturPlate Microplates
| Well format | 24-well | 384-well |
|---|---|---|
| Recommended working volume | 0.5-2.39 mL | 24 µL-90 µL |
| Height (mm) | 18.70 | 14.35 |
| Length (mm) | 127.80 | 127.76 |
| Width (mm) | 85.60 | 85.47 |
How much media can fit in a 6 well plate?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Media Volume (ml) | |
|---|---|---|
| 150 mm | 148 | 30-45 |
| 245 mm (square) | 500 | 100-150 |
| Dishes | ||
| 6 well | 9.5 | 1.9-2.0 |
How much media can a 6 well plate hold?
i.e. A well in a 6-well dishe has a surface area of 9.6cm2. The minimum volume should be 1.92ml and the maximum 4.8ml.
Why is 96 well plate used?
Transparent 96-Well Plates are more widely used, while Black 96-Well Plates are used when there is a specific need to minimize background in fluorescence reading. On the contrary, White 96-Well Plates are used to give maximum reflection and to minimize autofluorescence and autoluminescence.
What is the treatment plate?
Tissue culture treated plates offer a surface which enables most adherent cells to attach and proliferate.
What is TC surface?
“Cell culture-treated” usually refers to the process by which otherwise hydrophobic polystyrene surfaces are made to become hydrophilic, usually by increasing negative charge through a chemical means.
How many mL is a 6 well plate?
Growth Area of Tissue Culture Plates and Dishes
| Growth Area (cm2) | Media Volume (ml) | |
|---|---|---|
| 6 well | 9.5 | 1.9-2.0 |
| 12 well | 3.8 | 0.76-1.14 |
| 24 well | 1.9 | 0.38-0.57 |
| 48 well | 0.95 | 0.19-0.28 |
How much protein is in a 6 well plate?
80% confluency (35mm / 3.5cm) of a 6-well plate contains ~1e6 (1 million) cells / up to 300 µg cytoplasmic protein.
