Which provides higher resolution in MALDI-TOF?
Which provides higher resolution in MALDI-TOF?
Reflector
Explanation: Reflector provides higher resolution in MALDI-TOF.
What is spatial resolution in biology?
Spatial resolution refers to the minimum distance required to distinguish between two adjacent features and is a key parameter describing the performance of MSI techniques. The spatial resolution of MALDI MSI is often defined to be equal to the size of the laser spot1.
What is MALDI analysis?
Mass spectrometry is an analytical technique in which samples are ionized into charged molecules and ratio of their mass-to-charge (m/z) can be measured.
Is MALDI-TOF high resolution?
With regards to Mycoplasma species, MALDI-TOF MS has been used to identify clinically relevant human isolates [6, 7] as well as isolates from a variety of animal species, including M. bovis from cattle [6, 8,9,10,11]. Another laboratory tool is high-resolution melting PCR (HRM-PCR).
What does MALDI-TOF measure?
During MALDI-TOF analysis, the m/z ratio of an ion is measured by determining the time required for it to travel the length of the flight tube. A few TOF analyzers incorporate an ion mirror at the rear end of the flight tube, which serves to reflect back ions through the flight tube to a detector.
What is spatial resolution used for?
Spatial resolution can determine the quality of an image and describe how detailed an object can be represented by the image. It is a measurement to determine how small an object should be in order for an imaging system to detect it.
What is spatial and temporal resolution?
The spatial resolution is the amount of spatial detail in an observation, and the temporal resolution is the amount of temporal detail in an observation.
What is MALDI used for?
Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.
What is MALDI mass spec?
In mass spectrometry, matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation.
What does MALDI stand for?
Next Generation Bacterial Identification at WADDL MALDI-TOF stands for Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, an instrument that allows our microbiologists to quickly identify bacteria based on the organism’s protein composition.
