What is the transcriptome of a cell?
What is the transcriptome of a cell?
A transcriptome is a collection of all the gene readouts present in a cell.
What is 10x single-cell sequencing?
10x Genomics’ single-cell RNA-seq (scRNA-seq) technology, the Chromium™ Single Cell 3′ Solution, allows you to analyze transcriptomes on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries.
What is in a transcriptome?
A transcriptome is the full range of messenger RNA, or mRNA, molecules expressed by an organism. The term “transcriptome” can also be used to describe the array of mRNA transcripts produced in a particular cell or tissue type.
How does scRNA sequencing work?
The scRNA-Seq workflow is more or less similar to the scATAC-seq workflow except for the nuclei isolation step, since RNAs are located in the cytoplasm. Cells are sorted, RNAs are tagged with a barcode and reverse transcribed. All RNAs from the same cells are tagged with the same barcode.
What is smart Seq2?
Method Category: Transcriptome > RNA Low-Level Detection. Description: For Smart-Seq2, single cells are lysed in a buffer that contains free dNTPs and oligo(dT)-tailed oligonucleotides with a universal 5′-anchor sequence. RT is performed, which adds 2–5 untemplated nucleotides to the cDNA 3′ end.
How many cells are in 10X?
The optimal cell concentration for a 10X Genomics single cell RNA sequencing experiment is 400-1200 cells/µL in a minimal volume of 30-100 µL.
How many genes are in a cell 10X?
Genes detected per cell Seeing gene detection in the range of 500-5000 is normal for inDrop/10X analyses.
Why is the transcriptome important?
Understanding the transcriptome is essential for interpreting the functional elements of the genome and revealing the molecular constituents of cells and tissues, and also for understanding development and disease.
