How do you calculate WBC on a hemocytometer?

To count cells using a hemocytometer, add 15-20μl of cell suspension between the hemocytometer and cover glass using a P-20 Pipetman. The goal is to have roughly 100-200 cells/square. Count the number of cells in all four outer squares divide by four (the mean number of cells/square).

How is WBC count calculated?

Number of WBC in 1µL = Y x 10 x 20/4 = Y x 50 = Total WBC count. Total TLC = counted cells (Y) x 50 = TLC/cmm.

How do you calculate cells in a hemocytometer?

You can calculate your cell concentration using the following formula:

  1. Total cells/ml = (Total cells counted x Dilution factor x 10,000 cells/ml)/ Number of squares counted.
  2. Total cells/ml = (325 cells x 2 x 10,000 cells/ml)/ 5 = 130 x 104 cells/ml.
  3. Total cells in sample = 130 x 104 cells/ml x 5 ml = 650 x 104 cells.

Why do you multiply by 10000 when counting cells?

You can think of each large square as having a volume of 100nL. So you have to multiply by 10,000 in order to convert the number of cells in 100nL to the number of cells per mL.

What is total WBC count?

Normal Results. The normal number of WBCs in the blood is 4,500 to 11,000 WBCs per microliter (4.5 to 11.0 × 109/L). Normal value ranges may vary slightly among different labs. Some labs use different measurements or may test different specimens.

When counting white blood cells What is the volume of each square?

Let ‘n’ be the mean. Therefore, the average number of cells in each of the tiny 0.04 mm2 squares is n. The volume of each of these cells is 0.04 x 0.1 = 0.004 mm3.

How do you calculate WBC differential?

To determine the differential, a drop of blood is thinly spread over a glass slide, air dried, and stained with a Romanofsky stain, most commonly the Wright or May-Grunewald-Giemsa technique. Two hundred cells are then counted and classified.

What is the correct total WBC count quizlet?

The normal adult range for a white blood cell count is 4500 to 11,000.

What are the different types of WBC?

Types of white blood cells

  • Monocytes. They have a longer lifespan than many white blood cells and help to break down bacteria.
  • Lymphocytes. They create antibodies to fight against bacteria, viruses, and other potentially harmful invaders.
  • Neutrophils. They kill and digest bacteria and fungi.
  • Basophils.
  • Eosinophils.