Why is branch migration important?

Branch migration of Holliday junctions may serve several important functions such as affecting the length of genetic information transferred between homologous chromosomes during meiosis, restarting stalled replication forks, and ensuring the faithful repair of double strand DNA breaks by homologous recombination.

Does branch migration require ATP?

We show that RuvAB-mediated branch migration requires ATP and can bypass UV-induced DNA lesions. At high RuvB concentrations, the requirement for RuvA is overcome, indicating that the RuvB ATPase provides the motor force for branch migration.

What happens during meiotic recombination?

When recombination occurs during meiosis, the cell’s homologous chromosomes line up extremely close to one another. Then, the DNA strand within each chromosome breaks in the exact same location, leaving two free ends. Each end then crosses over into the other chromosome and forms a connection called a chiasma.

Which of the following promotes branch migration at higher rates than does rec A?

6. Which of the following promotes branch migration at higher rates than does Rec-A? Explanation: Ruv-A and Ruv-B proteins form a complex that binds to Holliday intermediates, displays Rec-A protein and promotes branch migration at higher rates than does Rec-A.

What is branch migration in recombination?

Branch migration is the second step of genetic recombination, following the exchange of two single strands of DNA between two homologous chromosomes. The process is random, and the branch point can be displaced in either direction on the strand, influencing the degree of which the genetic material is exchanged.

What is branch migration Mcq?

Explanation: Each time holiday junction moves, base pairs are broken in the parental DNA molecules and identical base pairs are created in the recombinants. This process of sliding of the holiday junction forming identical base pairs in the heteroduplex DNA is known as branch migration.

Which process does not occur during recombination?

Which process does not occur during recombination? DNA polymerization. Recombination does not include the synthesis of new DNA.

What happens during crossover and recombination in meiosis?

During meiosis, an event known as chromosomal crossing over sometimes occurs as a part of recombination. In this process, a region of one chromosome is exchanged for a region of another chromosome, thereby producing unique chromosomal combinations that further divide into haploid daughter cells.

Which processes result in recombination?

Genetic recombination happens as a result of the separation of genes that occurs during gamete formation in meiosis, the random uniting of these genes at fertilization, and the transfer of genes that takes place between chromosome pairs in a process known as crossing over.

Which recombination mechanism is operated during gene splicing?

Cre-Lox recombination involves the targeting of a specific sequence of DNA and splicing it with the help of an enzyme called Cre recombinase. Cre-Lox recombination is commonly used to circumvent embryonic lethality caused by systemic inactivation of many genes.

What is branch migration a break and reformation of identical base pairs?

What is branch migration? Explanation: Each time holiday junction moves, base pairs are broken in the parental DNA molecules and identical base pairs are created in the recombinants. This process of sliding of the holiday junction forming identical base pairs in the heteroduplex DNA is known as branch migration.