Why did the separation of pigments in the spinach extract occur?
Why did the separation of pigments in the spinach extract occur?
Pigments are separated according to differences in their relative solubilities. In order to extract these pigments from the thylakoid membranes of the chloroplasts, the organelles in which photosynthesis occurs, fresh, ground or torn leaves (preferably spinach) may be soaked in acetone or concentrated alcohol.
Why is the crushed spinach extracted initially with methanol and then with a hexane methanol mixture?
The extraction is performed by first crushing the leaves with methanol, and then with a mixture of methanol and hexanes (two immiscible solvents). The first extraction with methanol breaks the cell membranes and removes water.
How do you make spinach extract for chromatography?
1) Place in a mortar about 10 leave of fresh spinach, and combine them with 6 grams of sand and 2 grams of anhydrous magnesium sulfate. Add 2 mL of acetone. Using a pestle grind the mixture until a green solution is formed and most of the spinach has turned into a pulp.
How is beta-carotene extracted?
If it is desired to extract beta-carotene, the algae are first treated with calcium hydroxide and then filtered. The residue from this filtration is treated with a beta-carotene solvent which removes the beta-carotene from the residue and into the solvent.
Why is spinach used in chromatography?
Using spinach extract chromatography, the main plant pigments that will separate out are those that give the plant its dark green color. ​Chlorophylls​, which are plant pigments essential for the process of photosynthesis, are green pigments that are found in large quantities in spinach leaves.
Why did the separation of pigments in the spinach extract occur as it did how does paper chromatography work?
Why did the separation of pigments in the spinach extract occur as it did? How does paper chromatography work? The substances in the mixture dissolve in the alcohol and move up the paper. The heavier substances move up the paper more slowly.
What is the purpose of treating your spinach with methanol prior to extraction?
The polarity of these organic solvents makes them the most effective solvents for extraction and methanol gives the highest extraction yield as well as the highest content of bioactive compounds.
What role does Methanol play in the extraction Why was hexanes added to the extraction mixture?
The first extraction with methanol breaks the cell membranes and removes water. This extract is discarded because it is poor in pigments. The second extraction with methanol-hexanes removes the pigments that go preferentially to the hexane layer.
How do you extract pigment from spinach?
In part A, you will extract the chlorophyll and caretenoid pigments from spinach leaves using acetone as the solvent. The pigments will be separated by column chromatography using alumina as the adsorbent. Increasingly more polar solvents will be used to elute the various components from the column.
How do you make spinach leaf extract?
One-half bag of supermarket spinach leaves should be placed in a commercial blender with about 200 mL of water and ground thoroughly for 5-10 minutes, or until all of the leaves are blended. Split the extract into two and filter each using a large Buchner funnel into a 500-mL filter flask.
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