Which microorganisms are used for the production of protease through solid-state fermentation?

Which microorganisms are used for the production of protease through solid-state fermentation?

Abstract. Aspergillus oryzae MTCC 5341, when grown on wheat bran as substrate, produces several extracellular acid proteases. Production of the major acid protease (constituting 34% of the total) by solid-state fermentation is optimized.

How is protease produced?

Proteases are predominantly produced by microorganisms using submerged and solid-state fermentations. The bottleneck of production is found in downstream processes, which can account for 70 to 90% of the total production cost. Precipitation is used in addition to extraction using aqueous two-phase systems (ATPSs).

How do proteases produce enzymes?

Proteases are generally produced using submerged fermentation due to its apparent advantages in consistent enzyme production characteristics with defined medium and process conditions and advantages in downstream in spite of the cost-intensiveness for medium components [97].

What microorganisms are used in fermentation process?

The fermenting microorganisms mainly involve L.A.B. like Enterococcus, Streptococcus, Leuconostoc, Lactobacillus, and Pediococcus [6] and yeasts and molds viz. Debaryomyces, Kluyveromyces, Saccharomyces, Geotrichium, Mucor, Penicillium, and Rhizopus species [7–10].

Which microorganisms helpful in fermentation process?

Fermented foods are preserved by the production of citric, lactic, or acetic acids by beneficial bacteria such as Lactobacillus, Streptococcus, Bacillus, and Pseudomonas, yeasts, and fungi that use the food as a substrate for their growth and metabolism.

Where are protease produced?

Protease. Protease is produced in the stomach, pancreas, and small intestine. Most of the chemical reactions occur in the stomach and small intestine. In the stomach, pepsin is the main digestive enzyme attacking proteins.

Where is protease created?

Protease enzymes are produced in your stomach, pancreas and small intestine.

What is solid-state fermentation example?

Examples include fermented bakery products such as bread or for the maturing of cheese. SSF is also widely used to prepare raw materials such as chocolate and coffee; typically cacao bean fermentation and coffee bean skin removal are SSF processes carried out under natural tropical conditions.

What is the advantage of solid-state fermentation?

Advantages of Solid State Fermentation (SSF) Solid substrate fermentation employs simple natural solids as the media. Low technology, low energy expenditure and requires less capital investment. No need for sterilization, less microbial contamination, and easy downstream processing.

Where is protease enzyme produced?

Protease is produced in the stomach, pancreas, and small intestine. Most of the chemical reactions occur in the stomach and small intestine. In the stomach, pepsin is the main digestive enzyme attacking proteins. Several other pancreatic enzymes go to work when protein molecules reach the small intestine.

What is the product of protease?

Protein Amino acids
Where enzymes are produced