Which material is used in HPLC?

HPLC columns are usually packed with pellicular or porous particles. Pellicular particles are made from polymer or glass beads. These beads have a diameter between 30 and 40 µm. They are surrounded by a thin uniform layer of silica, alumina, or other types of ion-exchange resins.

What is L3 packing in HPLC column?

L3 USP columns are described as consisting of porous silica particles, 1.5 to 10 µm in diameter. Our L3 USP columns are produced to the highest quality standards to ensure reliable, reproducible results.

What is L1 packing for HPLC column?

According to the United States Pharmacopeia (USP), L1 column packing is defined as “octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 m in diameter.” Some USP methods specifying an L1 column use intermediate pH, and many of these methods were done on a 10 m, 3.9 x 300 mm column.

What is packing material in chromatography?

Packing Material Pellicular particles are made from polymer, or glass beads. Pellicular particles are surrounded by a thin uniform layer of silica, polystyrene-divinyl-benzene synthetic resin, alumina, or other type of ion-exchange resin. The diameter of the pellicular beads is between 30 and 40 µm.

Which of the following polymer polymers are widely used in affinity chromatography?

In a typical affinity chromatography experiment, the ligand is attached to a solid, insoluble matrix—usually a polymer such as agarose or polyacrylamide—chemically modified to introduce reactive functional groups with which the ligand can react, forming stable covalent bonds.

What is BDS column?

BDS column is a type of reverse-phase HPLC column which has blocked –OH groups. This means the hydroxyl groups in this column are deactivated/not free. It is also named as BDS C18 column, and this column is packed with octadecasilane chains. The term BDS stands for Base Deactivated Silica.