Where does the PstI restriction enzyme cut?
Where does the PstI restriction enzyme cut?
Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
What type of restriction enzyme is BsaI?
BsaI has a High Fidelity version BsaI-HF®v2 (NEB #R3733). High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
What is single cutter restriction enzymes?
Restriction enzymes are DNA-cutting enzymes. Each enzyme recognizes one or a few target sequences and cuts DNA at or near those sequences. Many restriction enzymes make staggered cuts, producing ends with single-stranded DNA overhangs. However, some produce blunt ends.
What does PstI stand for?
PSTI
| Acronym | Definition |
|---|---|
| PSTI | Patient Specific Therapeutic Interchange |
| PSTI | Precise Software Technologies Inc (software developers; Ottawa, Ontario, Canada; est. 1989) |
| PSTI | Petroleum Science and Technology Institute (UK) |
| PSTI | PS Technologies, Inc. |
How many fragments are produced by PstI?
two DNA
c) For those two restriction endonucleases, each DNA fragment in the single digest is cut by PstI into two DNA fragments in the double digest (i.e. the restriction endonuclease plus PstI).
How does BsaI cut?
Type IIS restriction enzymes cut DNA at a location adjacent to their non-palindromic recognition site. For example BsaI creates a 4 nucleotide 3′-recessed overhang adjacent to the recognition site (See Figure below). The overhang can comprise any nucleotides, which allows BsaI to create 256 unique overhangs.
What is DCM methylation?
The Dcm methylase (encoded by the dcm gene; referred to as the Mec methylase in earlier references) methylates the internal (second) cytosine residues in the sequences CCAGG and CCTGG (1,3) at the C5 position. The EcoKI methylase, M. EcoKI, modifies adenine residues in the sequences AAC(N6)GTGC and GCAC(N6)GTT.
What is the use of NEB cutter?
NEBcutter — A restriction analysis tool Find restriction digestion map of a DNA sequence. Highlights: This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E. coli genetic code and the sites for all Type II restriction enzymes that cut the sequence just once.
