What was the wavelength of light used in the spectrophotometer?

A UV-visible spectrophotometer uses light over the ultraviolet range (185 – 400 nm) and visible range (400 – 700 nm) of the electromagnetic radiation spectrum.

What are spectroscopes used to measure?

A spectroscope is a device that measures the spectrum of light. Early versions had a slit, a prism, and a screen with markings to indicate various wavelengths or frequencies; later versions were calibrated to electronic detectors.

How does spectrometer work?

The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample.

What does a spectrophotometer detect?

The detector detects how much light was reflected from or transmitted through the sample. The detector then converts how much light the sample transmitted or reflected into a number.

Where are spectroscopes used?

astronomy
Spectroscopes are often used in astronomy and some branches of chemistry. Early spectroscopes were simply prisms with graduations marking wavelengths of light. Modern spectroscopes generally use a diffraction grating, a movable slit, and some kind of photodetector, all automated and controlled by a computer.

What are the application of spectrometer?

Uses of Spectrometer Monitoring dissolved oxygen content in freshwater and marine ecosystems. Studying spectral emission lines of distant galaxies. Characterization of proteins. Space exploration.

Why do we use Spectrophotometry?

Spectrophotometry is a measurement of how much a chemical substance absorbs or transmits. Spectrophotometry is widely used for quantitative analysis in various areas (e.g., chemistry, physics, biology, biochemistry, material and chemical engineering, clinical applications, industrial applications, etc).

What is the application of spectrophotometer?

Applications of Spectrophotometer A spectrophotometer is used to know the concentration of solutes colorless or coloured in a solution. It is used for the determination of the rate of reaction by measuring the rate of formation and disappearance. It is used in forensic sciences. It is used in molecular biology.

Which light source is used in spectroscopy?

Two kinds of lamps, a Deuterium for measurement in the ultraviolet range and a tungsten lamp for measurement in the visible and near-infrared ranges, are used as the light sources of a spectrophotometer.