What type of microscope can see mitochondria?

light microscopes
Thus, light microscopes allow one to visualize cells and their larger components such as nuclei, nucleoli, secretory granules, lysosomes, and large mitochondria. The electron microscope is necessary to see smaller organelles like ribosomes, macromolecular assemblies, and macromolecules.

What does mitochondria look like under a microscope?

Mitochondria have a distinctive appearance when viewed by electron microscopy. They often appear as rounded or sausage-shaped structures (Figure 1a, b and Figure 22a, b), measuring about 0.5-1.0 µm in diameter and 2-8 µm in length; although their size and shape vary, and they are often much bigger in plants.

Can you see mitochondria with light microscope?

Mitochondria are visible with the light microscope but can’t be seen in detail.

Can mitochondria be seen with a light microscope?

Mitochondria are visible with the light microscope but can’t be seen in detail. Ribosomes are only visible with the electron microscope.

Can light microscopy see cristae?

In fixed cells, cristae were first visualized by light microscopy with isoSTED nanoscopy9.

What stain is used to observe mitochondria?

Janus green
Janus green is a basic dye and vital stain used for staining the mitochondria of a cell.

What stain is used for mitochondria?

DASPEI (2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) is used to stain mitochondria in live cells. DASPEI (2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) is used to stain mitochondria in live cells.

Is mitochondria visible under light microscope?

Can storm microscopy be used to monitor mitochondrial dynamics?

Using STORM microscopy with a vicinal-dithiol-proteins targeting probe, visualizing mitochondrial dynamics was attainable with spatial and temporal resolutions of 45 nm and 0.8 s, notably, dynamic mitochondrial tubulation retraction of ~746 nm in 1.2 s was monitored.

Is there a time-lapse STORM image of the mitochondria?

Although SMLM has mostly been performed on fixed mitochondria, Shim et al. demonstrated time-lapse STORM images of BS-C-1 cells labeled with MitoTracker Red, which highlights the inner membrane (61). Their recordings revealed thin, extended tubular intermediates connecting neighboring mitochondria both prior to fission and after fusion (Fig. 4B).

What is a storm microscope?

STORM microscopes can produce high-quality images of cellular components resolved to under 20 nm, changing the way scientists visualize molecular structures, interactions within organelles and biological processes.

Can expansion microscopy be used to study mitochondria?

Indeed, mitochondria were among the first organelles analyzed with expansion microscopy, achieving a detailed imaging of membrane protein distributions and cristae structures (39). Still, to our knowledge, expansion microscopy has not been used to address questions of mitochondrial biology, yet.