What temperature does Serratia marcescens grow best at?
What temperature does Serratia marcescens grow best at?
25-37C
Perhaps it is noteworthy that the optimal growth range for Serratia marcescens is 25-37C, and it is above this temperature range that pigment production ceases. It is possible that the loss of pigment is associated with mild temperature stress.
Is prodigiosin sensitive to temperature?
The paper above does state that temperature does effect prodigiosin expression. Such that at higher temperatures a physiological response is invoked rather than a genetic change in prodigiosin expression. Besides temperature, pH and nutrients are important in prodigiosin expression (pigment expression).
What is prodigiosin used for?
Prodigiosin received renewed attention for its wide range of biological activities, including activities as antimalarial, antifungal, immunosuppressant, and antibiotic agents. It is perhaps best known for its capacity to trigger apoptosis of malignant cancer cells.
What is the function of prodigiosin in Serratia marcescens?
Prodigiosin is a red pigment produced by Serratia marcescens. Prodigiosin is regarded as a promising drug owing to its reported characteristics of possessing anti-microbial, anti-cancer, and immunosuppressive activity.
Why do we incubate Serratia marcescens at room temperature 25 C instead of 37 C?
– 25°C was the temperature that allowed for optimal pigment production. – Serratia marcescens grew a red pigment on the surface of the slant. -There was indeed prodigiosin production in this result. Draw and discuss the test tube incubated at 25°C.
What effect does temperature have on Serratia?
The pigment in Serratia marcescens is called prodigiosin. Once the growth temperature of Serratia marcescens is raised to 37 degrees, the pigment stops being produced. It is believed that an enzyme used in the production of prodigiosin is affected by the temperature so that the pigment is no longer made.
At what temperature does S marcescens produces prodigiosin pigmented metabolite?
Williams et al. (1971) defined 27°C as the optimum temperature for prodigiosin production in S. marcescens, and that the pigment is synthesized during the stationary phase of growth.
What is Violacein used for?
As a compound, violacein is known to have diverse biological activities, including being an anticancer agent and being an antibiotic against Staphylococcus aureus and other Gram-positive pathogens.
Is prodigiosin a protein?
A protein associated to prodigiosin formation was found in Serratia marcescens. The protein was not found in nonpigmented strains and was correlated with the pigment level.
Where is prodigiosin found?
5.11 PRODIGIOSIN They are typically produced on starch-rich foods such as bread, and their formation has often been interpreted as a religious omen (Fuerstner, 2003). Members of this family are produced by microorganisms such as Serratia marescensa and Streptomyces.
At what temperature does S. Marcescens produce prodigiosin pigment metabolite?
Why does S. Marcescens produce prodigiosin?
In this model, as the Serratia, population size increases, their gene expression pattern changes accordingly. One of the consequences of this is that some new metabolic pathways are initiated, a byproduct of which is the prodigiosin pigment.
