What protein is expressed by POU5F1?

The Oct4 protein, encoded by the Pou5f1 gene was the very first master gene, discovered 25 years ago, to be absolutely required for the stemness properties of murine and primate embryonic stem cells.

What does the POU5F1 gene do?

The POU5F1 transcription factor is the gatekeeper of the pluripotent state in mammals. It is essential for epigenetic reprogramming events and also for germ cell viability. The Pou5f1 gene’s expression is tightly controlled during embryogenesis, but its regulatory regions are not fully deciphered.

What is the function of Oct4?

Oct4 mainly functions through the activation of pluripotency-associated and self-renewal-associated genes, while simultaneously repressing genes that promote differentiation, in coordination with other pluripotency factors and coregulators.

What does Oct4 stand for?

octamer-binding transcription factor 4
Oct-4 (octamer-binding transcription factor 4), also known as POU5F1 (POU domain, class 5, transcription factor 1), is a protein that in humans is encoded by the POU5F1 gene. Oct-4 is a homeodomain transcription factor of the POU family.

What is NANOG in biology?

NANOG is a transcription factor in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG is thought to function in concert with other factors such as POU5F1 (Oct-4) and SOX2 to establish ESC identity.

Is SOX2 a protein?

Sox2 is a member of the Sox family of transcription factors, which have been shown to play key roles in many stages of mammalian development. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids.

What is Nanog gene?

NANOG is a transcription factor that is involved in the self-renewal of embryonic stem cells (ES) and is a critical factor for the maintenance of the undifferentiated state of pluripotent cells.

What is the role of Oct4 in embryonic stem cells?

Oct4 is a key component of the pluripotency regulatory network, and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells (ESCs) or their differentiation into trophoblast.

What is the significance of NANOG?

NANOG protein expression is a biomarker that indicates poor clinical outcome in lung, breast, gastric, colorectal, pancreatic, and ovarian cancer as well as in hepatocellular, oral squamous cell, esophageal, and nasopharyngeal carcinoma.

Where is NANOG found?

The human Nanog 1 gene is located on chromosome 12, and the mRNA contains a 915 bp open reading frame (ORF) with 4 exons and 3 introns. The N-terminal region of human NANOG is rich in serine, threonine and proline residues, and the C-terminus contains a tryptophan-rich domain.