What is the use of NanoDrop spectrophotometer?
What is the use of NanoDrop spectrophotometer?
The NanoDrop® ND-1000 UV-Vis Spectrophotometer is a spectrophotometer which enables highly accurate analyses of 1 ul samples for DNA, RNA, protein, pigments, whole uv-visible (220-700nm) spectrum and cell density tests with remarkable reproducibility.
What information does NanoDrop measure?
A: The NanoDrop Lite is designed to measure the absorbance and calculate the concentration of nucleic acids (260 nm) and purified proteins(280 nm). This would include dsDNA, ssDNA, RNA and purified proteins.
What are the two path lengths the NanoDrop 8000 measures?
1mm and 0.2 mm
The pathlengths are automatically controlled to 1mm and 0.2 mm paths.
How much does a NanoDrop cost?
| Catalog Number | Mfr. No. | Price |
|---|---|---|
| Catalog Number | Mfr. No. | Price |
| NDNDLUSCAN Thermo Scientific™ NanoDrop™ Lite Spectrophotometer viewProductCertificates | Thermo Scientific™ 840-281400 | Each for $6,160.00 |
What is the advantage of NanoDrop?
The main advantage the Nanodrop has over the Qubit is the ability to measure the purity of the sample. The 260/280 and 260/230 ratios give an indication of how pure the sample is from protein and salt contaminants.
How does NanoDrop measure DNA?
If using a NanoDrop to measure your samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample.
How does a spectrophotometer work?
A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample.
How do you clean a Nanodrop plate?
Apply 5 ul of dH20 solution to the bottom pedestal. Lower the upper pedestal arm to form a liquid column; let it sit for approximately 2-3 minutes. Wipe away the water from both the upper and lower pedestals with a clean lab wipe.
What is the range of NanoDrop?
NanoDrop 2000/2000c Spectrophotometers
| Absorbance Accuracy | 3% (at 0.74 Abs at 350 nm) |
|---|---|
| Wavelength Range | 190 to 840 nm |
| Footprint | 14 x 20 cm |
| For Use With (Application) | Nucleic Acid Quantification, DNA Quantification, RNA Quantification, Protein Quantification |
| Includes | Laptop Computer |
How does NanoDrop measure DNA concentration?
How does NanoDrop measure concentration?
Using the absorbance at 280nm (A280), protein concentration (c) is calculated using the Beer-Lambert equation A280 = c * ε * b (ε is the wavelength-dependent protein extinction coefficient, b is the pathlength). Each pure protein has a unique extinction coefficient.
