What is the principle of kinyoun staining?

The primary stain for the Kinyoun procedure is the aniline dye, basic fuchsin, that stains all the cells present red. The unique ability of mycobacteria to resist decolorization by acid-alcohol is why they are termed acid-fast, and will keep their red coloration throughout the staining process.

What is Ziehl-Neelsen stain used for?

Conventional smear microscopy with the Ziehl-Neelsen (ZN) stain is a rapid and practical method for detecting acid-fast bacilli (AFB), especially in low-income countries, due to its rapidity, low cost, and high positive predictive value for tuberculosis (14).

What is the difference between Ziehl-Neelsen and Kinyoun stain?

Unlike the Ziehl–Neelsen stain (Z-N stain), the Kinyoun method of staining does not require heating. In the Ziehl–Neelsen stain, heat acts as a physical mordant while phenol (carbol of carbol fuschin) acts as the chemical mordant.

What is the secondary stain in Kinyoun method?

Principle of Kinyoun-Cold Method The excess stain is removed with treatment by 1% sulfuric acid. A secondary stain, methylene blue, is then applied to the cells.

What is the advantage of the kinyoun staining procedure?

What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method? a complex lipid that is composed of fatty acids and fatty alcohols that have hydrocarbon chains up to 80 carbons in length. the application of heat to cells during staining with carbolfushcin and phenol is not without safety concern.

What is the counterstain of Kinyoun?

methylene blue
Kinyoun staining method uses carbol-fuchsin as a primary stain, followed by decolorization with an acid-alcohol solution and methylene blue as a counterstain. Kinyoun carbol-fuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.

What stain is used for tuberculosis?

Ziehl-Neelsen (ZN) stain
Sputum smear microscopy using the ubiquitous Ziehl-Neelsen (ZN) stain, the primary diagnostic strategy for active tuberculosis (TB) worldwide that is recommended by the World Health Organization (WHO),1 is constrained by its reliance on human skill and time-intensive nature.

What is the difference between Gram staining and Ziehl Neelsen staining?

An acid-fast stain is able to differentiate two types of gram-positive cells: those that have waxy mycolic acids in their cell walls, and those that do not. Two different methods for acid-fast staining are the Ziehl-Neelsen technique and the Kinyoun technique. Both use carbolfuchsin as the primary stain.

Which stain is more sensitive Kinyoun or Fluorochrome?

Fluorochrome stains are more sensitive, though not as specific, but may be useful for screening of the tissue specimens. These include auramine-O (Fig. 11.10) and the rhodamine stains. Organisms visualized using these stains should be confirmed by a Ziehl-Neelsen or Kinyoun stain.

What is Fluorochrome staining?

Fluorochrome staining: The use of any fluorescent dye (e.g., auramine, rhodamine) used to label or stain. Must be viewed using a fluorescence microscope.

What is the wetting agent used in Kinyoun method?

The wetting agent, tergitol, reduces surface tension between the cell wall of the mycobacteria and the stain allowing it to penetrate. Why is heat or a surface-active agent used with the application of the primary stain during acid-fast staining?