What is the difference between pET28a and pet28b?

pdf): “The maps for pET-28b(+) and pET-28c(+) are the same as pET-28a(+) (shown) with the following exceptions: pET-28b(+) is a 5368bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-28c(+) is a 5367bp plasmid; subtract 2bp from each site beyond BamH I at 198.”

What is pet28?

pET28a is the most popular expression plasmid on the market (described in >40,000 published articles). It contains the T7 promoter and an adjacent lac operator sequence that is included to suppress uninduced expression5.

What is pGEX vector?

pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.

What is a pet9a vector?

pET-9a is a 4341bp vector for cloning and expression. pET vectors pET-9a-d(+) possess an N-terminal T7•Tag and a MCS that includes a BamHI cloning site.

How does the pET expression system work?

The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals, expression is induced by providing a source of T7 RNA …

How does tac promoter work?

The tac promoter is used to control and increase the expression levels of a target gene and is used in the over-expression of recombinant proteins. The tac promoter is named after the two promoters which comprise its sequence: the ‘trp’ and the ‘lac’ promoters.

What is DE3 and BL21?

BL21(DE3) is an E. coli B strain and does not contain the lon protease. It is also deficient in the outer membrane protease OmpT. The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells. • High transformation efficiency: >1×107 cfu/µg pUC19 DNA.