What is tdTomato?

tdTomato is an exceptionally bright red fluorescent protein—6X brighter than EGFP. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. The tdTomato fluorescent protein is as photostable as mCherry (Shaner et al. 2004).

Is tdTomato the same as mCherry?

The tandem dimer tdTomato is equally photostable but twice the molecular weight of mCherry, and may be used when fusion tag size does not interfere with protein function. mStrawberry is the brightest red monomer, but it is less photostable than mCherry, and should be avoided when photostability is critical.

Where is tdTomato expressed?

TdTomato is expressed in numerous GABAergic neurons within these brain regions and is visible in somata, dendrites, as well as axons. The scale bar corresponds to 50 μm and applies to all panels. A distinct, but less intense expression of tdTomato was observed in the cerebellum.

Where does red fluorescent protein come from?

Whereas many fluorescent proteins are found in sea creatures, far-red and near-infrared molecules tend to come from bacteria.

Is RFP the same as tdTomato?

Red fluorescent protein (RFP) is a biological marker used for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP comes in many forms including tdTomato, mCherry, mStrawberry, and DsRed and produces a bright red fluorescence when exposed to blue light.

How are fluorescent proteins used?

Photoactivatable fluorescent proteins enable tracking of photolabeled molecules and cells in space and time and can also be used for super-resolution imaging. Genetically encoded sensors make it possible to monitor the activity of enzymes and the concentrations of various analytes.

What channel is mCherry?

It’ll depend on the flow cytometer you have access to but to best detect the mCherry signal excite with the yellow-green laser at 561 nm and detect in the PE-TexasRed channel with a 610/20 bandpass filter.

What is red fluorescent protein used for?

Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.