What is TapeStation used for?

TapeStation Systems The Agilent TapeStation system is an automated electrophoresis solution for the sample quality control of DNA and RNA samples. The system integrates an instrument, data processing software, reagents, and ScreenTape devices specific for DNA and RNA.

What is the difference between TapeStation and Bioanalyzer?

A: The Agilent 2200 TapeStation system can run anywhere from 1 to 95 samples. The Bioanalyzer is “chip- based” and can only fit 11 or 12 samples per run. Therefore, the service fee for Bioanalyzer run is charged on a per chip basis. Both Bioanalyzer and TapeStation can run cDNA or NextGen library samples.

What is a good DNA integrity number?

01.05 and higher) assesses the integrity of gDNA samples and assigns the DNA Integrity Number (DIN), a numerical measure of the gDNA integrity. This value ranges from 1 to 10, where 1 indicates highly degraded gDNA and 10 represents highly intact gDNA3.

How is DNA integrity number calculated?

In order to standardize this assessment, a novel algorithm was developed to score gDNA samples on the 2200 TapeStation system. The DNA integrity number (DIN) is calculated from several features obtained from the electrophoretic trace and provides a numerical value from 1 (degraded) to 10 (intact).

How does TapeStation calculate concentration?

For D1000 ScreenTape and High Sensitivity D1000 ScreenTape, the concentration values are calculated using the area of the sample peak compared to the known concentration of the top marker. It is, therefore, essential that all peaks are integrated correctly in the 2200 TapeStation Analysis Software.

How is DIN calculated Tapestation?

DIN determines the fragmentation of a genomic DNA sample by assessing the distribution of signal across the size range, and applies an automatically calculated number. DIN was generated using approximately 7,000 samples from a wide variety of genomic DNA sources comparing the signal against each and every sample.

What does DNA integrity mean?

DNA integrity is defined as the absence of both single strand or double strand and breaks absence of nucleotide modifications in the DNA [44].

What is RIN value?

RIN values range from 10 (intact) to 1 (totally degraded). The gradual degradation of rRNA is reflected by a continuous shift towards shorter fragment sizes.

Is TapeStation capillary electrophoresis?

The Agilent TapeStation system is an automated electrophoresis solution for the sample quality control of DNA and RNA samples.