What is Prestained protein ladder?

Prestained Protein Ladder, Broad Range (10-230 kDa) is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity.

What is the kDa ladder?

This unstained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

What is protein ladder used for?

Protein ladders or molecular weight markers are among the most commonly used reagents in biochemistry experiments. They provide molecular weight standards to estimate the size of proteins separated by gel electrophoresis like SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).

What is the ladder in Western blot?

Western blot protein ladders are a mixture of pre-stained, StrepTactin-HRP Conjugate proteins (or Strep-tagged recombinant proteins). It enables fluorescent visualization of protein gels and chemiluminescent or colorimetric immunodetection of western blots.

What is unstained protein ladder?

Unstained Protein Ladder is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-250 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 (1,2).

What is the purpose of a DNA ladder?

DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories.

How much protein is in a ladder?

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Why is protein ladder used in SDS-PAGE?

Protein ladders, also known as protein markers or protein standards, are used to help estimate the size of proteins separated during electrophoresis. They serve as points of reference because they contain mixtures of highly purified proteins with known molecular weights and characteristics.

How do you stain a unstained protein ladder?

If you want to do with unstained ladder yet, I recommend to do all steps of western blot just routinely do. After transferring the protein bands onto the blot paper, stain the paper with Ponceau-S for visualization of ladder. Then, cut a small piece of the paper standing in front of your desired protein band.