What is Petroff-Hausser?

The Petroff-Hausser Counter is one piece construction ensuring durability and accuracy, featuring an Improved Neubauer ruling on a single plateau. The Petroff-Hausser Counting Chamber is popular for bacteria and sperm counting and offered in a series of cell-depths(10, 20, 40, microns).

How does Petroff-Hausser counting chamber work?

Petroff-Hausser counting chambers can be used as a direct method to determine the number of bacterial cells in a culture or liquid medium. In this procedure, the number of cells in a given volume of culture liquid is counted directly in 10-20 microscope fields.

What is Neubauer chamber?

The Neubauer chamber is a thick crystal slide with the size of a glass slide (30 x 70 mm and 4 mm thickness). In a simple counting chamber, the central area is where the cell counts are performed.

What is direct microscopic count?

Direct Microscopic Count (DMC) is a quantitative test and is helpful in assessing the actual number of bacteria present in milk. DMC is used as a platform test to assess the microbiological quality of milk received at the Raw Milk Receiving Dock.

What is the most important reason why a Petroff Hausser chamber can be used to count bacteria?

Both can be used for cell count depending on availability in your lab. But, take into consideration the depth of the chamber you are using when calculating. The Petroff-Hausser chamber depth is 0.02 mm (much less), that’s why is used to count bacteria and sperm.

What is WBC diluting fluid?

WBC diluting fluid is used for perfoming the WBC (Leucocyte) count. Glacial acetic acid lyses the red cells. Gentian violet slightly stains the nuclei of the leucocytes.

How do you count bacteria?

Calculate the number of bacteria (CFU) per milliliter or gram of sample by dividing the number of colonies by the dilution factor multiplied by the amount of specimen added to liquefied agar.

When would you use direct microscopic count?

Direct Microscopic count (DMC) is a quantitative test and used to enumerate the number of bacterial clumps or somatic cells present in milk. This method is also used for the analysis of foods, water, and, in some cases, air for quantitative counting of microorganisms.