What is nuclease free water used for?

Overview. Nuclease-Free Water is used in various molecular biology applications requiring nuclease-free conditions, such as in processing DNA or RNA (i.e. PCR, cDNA synthesis, or qPCR). This product is sterile-filtered (0.2 µm), free of RNase and DNase activity, endotoxin-free, and not DEPC-treated.

Is nuclease free water a PCR grade?

Nuclease-free water is an essential component of all PCR-based molecular biology work.

Does nuclease free water expire?

Expiration: 10 x 2 mL Nuclease Free Water has a shelf life of up to 2 years from analysis when stored in a sealed container in ambient conditions.

Is nuclease free water RNase free?

As mentioned by Ganapati, nuclease free water is a water free of RNase and DNase.To obtain nuclease-free water you will need to treat your water with 0.1% DEPC (diethyl pyrocarbonate-Sigma, cat # D5758, stored @ 4˚C).

What is the pH of nuclease free water?

between 5.0 and 6.5
At 22°C, Nuclease-Free Water has a pH value of between 5.0 and 6.5.

Why do we use nuclease free water in PCR?

PCR and related PCR-based techniques, including quantitative PCR and reverse transcriptase PCR , require nuclease-free water to avoid the degradation of the nucleic acid.

Is HPLC grade water nuclease free?

Most freshly opened bottles of commercial HPLC grade water are already RNAse free. However, always test untreated water (or old treated water) for nucleases by incubating sample RNA in the water at 37°C for at least 3 hours and then run a gel to detect degradation.

How is nuclease free water made?

Popular Answers (1)

  • Get MilliQ (reverse osmosis purified) water.
  • Add 1 ml DEPC (Diethylpyrocarbonate) per 1000 ml of MilliQ or double distilled water (i.e. to a final concentration 0.1%) and mix thoroughly.
  • Let the DEPC-mixed water incubate for 12 hours at 37°C.
  • Autoclave DEPC-mixed water for 15 minutes.

Why are nucleases important?

DNA nucleases catalyze the cleavage of phosphodiester bonds. These enzymes play crucial roles in various DNA repair processes, which involve DNA replication, base excision repair, nucleotide excision repair, mismatch repair, and double strand break repair.

What are two types of nucleases what are their function?

What Are The Two Different Types Of Nucleases?

  • Endonucleases – they can break the internal phosphodiester bonds inside a molecule of DNA.
  • Exonucleases – eliminates nucleotides one at a time from the end of a DNA molecule.

Is nuclease free water distilled?

Yes, QIAGEN’s Nuclease-Free Water is distilled water that is completely free of substances that may fluoresce.