What is multi locus probe?

A probe that hybridizes to a number of different sites in the genome of an organism.

Why is a multi locus probe better than a single locus probe?

Multilocus probes detect in general short tandem repeats (STRs) spread throughout the entire genome while the information obtained using single locus probes is restricted to a minute spot in the large genome with the advantage of detecting both alleles.

What are single locus probes?

A single locus probe is a DNA or RNA sequence that is able to hybridize (i.e. form a DNA-DNA or DNA-RNA duplex) with DNA from a specific restriction fragment on the Southern blot. Duplex formation depends on complementary base pairing between the DNA on the Southern blot and the probe sequence.

What is the meaning of single locus?

In genetics, a locus (plural loci) is a specific, fixed position on a chromosome where a particular gene or genetic marker is located.

What is Mlst used for?

Multilocus sequence typing (MLST) is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes.

What are the Multilocus probes used in DNA fingerprinting?

The “classical” multilocus DNA probes, developed in the laboratory of Alec Jef- freys, named 33.6 and 33.15, are derived from a human minisatellite [2]. In the fragment size window usually chosen for these systems, an average of 17 variable DNA bands per individual is produced.

What did Mendel called genes?

Mendel (1866) proposed that inheritance is controlled by paired germinal units or factors, now called genes. These represent small segments of chromosome, i.e. chromosomal basis of heredity.

Which of the following is not a part of the methods used in single locus probe analysis of VNTR regions of human DNA?

Which of the following is NOT a part of the methods used in single locus probe analysis of VNTR regions of human DNA? Recombinant DNA is not used in forensic DNA profile analysis.

How is MLST performed?

The principle of MLST is simple: the technique involves PCR amplification followed by DNA sequencing. Nucleotide differences between strains can be checked at a variable number of genes depending on the degree of discrimination desired.

What is the difference between MLST and MLSA?

Moreover, MLST is usually applied to strains that belong to a well-defined species while MLSA is more often used when species boundaries are not well known and MLSA data are used to improve species descriptions.

How is an STR different from a VNTR?

VNTR consists of comparatively a long repeating units of nucleotides (10-60 base pairs). STR consists of short repeating units of nucleotides (2-6 bp). The main difference between VNTR and STR is the length of the repeating units of each type of tandem repeats.