What is Maldi imaging mass spectrometry?

MALDI Imaging is a powerful mass spectrometry tool for mapping the distribution of molecules from a thin sample, ranging from small metabolites to large proteins, without molecular tags or labels.

Why is MALDI used for imaging?

Because of its ability to perform molecular analyses while retaining morphological information, MALDI Imaging is very well suited to study molecular distribution patterns within a tumor.

What is MALDI MS used for?

Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.

How does MALDI MSI work?

MALDI imaging works through the utilization of a matrix, an acidic aromatic molecule that absorbs energy of the same wavelength produced by the irradiating laser.

How does mass spectrometry imaging work?

This technique is performed using a focused ionization beam to analyze a specific region of the sample by generating a mass spectrum. The mass spectrum is stored along with the spatial coordination where the measurement took place. Then, a new region is selected and analyzed by moving the sample or the ionization beam.

What is a MALDI test?

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) is a powerful analytical mass spectrometry technique that has generated numerous diagnostic and clinical applications, especially for the identification of microorganisms for medical diagnosis.

What is MALDI identification?

The MALDI Biotyper® (MBT) is a microbial identification system based on MALDI-TOF mass spectrometry, allowing unbiased identification of microorganisms within a few minutes down to the species level.

What is the basic principle of mass spectrometry?

“The basic principle of mass spectrometry (MS) is to generate ions from either inorganic or organic compounds by any suitable method, to separate these ions by their mass-to-charge ratio (m/z) and to detect them qualitatively and quantitatively by their respective m/z and abundance.

What are the advantages and disadvantages of mass spectrometry?

Pros and Cons It is an excellent tool for identifying unknown components in a sample or confirming their presence. Disadvantages of mass spec are that it isn’t very good at identifying hydrocarbons that produce similar ions and it’s unable to tell optical and geometrical isomers apart.