What is Jurkat T cell?

Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.

What kind of T cells are Jurkat cells?

Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. The original Jurkat clone was designated JM*. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available.

Is Jurkat CD4 or CD8?

CD4+
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.

What are Jurkat cells used for?

Jurkat cells are used most significantly to study T-cell Leukaemia, HIV and certain cancers. Jurkat and their derivative cell strains are immortal human T lymphocytes. They are possibly the best-known T-cell line making them a significant cell line.

Why are they called Jurkat cells?

Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called “JM” (JURKAT and JM are derived from the same patient and are sister clones).

How do you activate Jurkat T cells?

Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works.

What is the HLA type of Jurkat cells?

The Jurkat HLA panel is comprised of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.

Are Jurkat cells CD4?

Jurkat is a CD4 T cell line provided by O. Acuto (Institut Pasteur, Paris, France). Kit 225 is a CD4 T cell line expressing high- and intermediate-affinity IL-2R (13). YT is a cell line expressing intermediate-affinity IL-2R.

Does Jurkat express CD8?

All Answers (1) The Jurkat T cell leukemic line E6–1 expresses high levels of mature TCR and low levels of CD4, but no CD8 or MHC class II.

What does IL-2 Do to T cells?

IL-2 plays a dual role in T cell activation by stimulating the proliferation and differentiation of ‘conventional’ T cells as well as maintaining and expanding the population of ‘suppressive’ Treg cells (Fig.

Is Jurkat a CD4?

Are Jurkat cells activated?

Jurkat T cells were activated by anti-CD3, anti-CD28, PMA, or ionomycin or combinations of these single stimuli, in order to map the contribution of these stimuli towards the activation of proximal, medial and distal signal transduction pathways.