What is interstrand crosslink repair?

Interstrand crosslinks (ICLs) can block the progression of the replication fork by inhibiting the progression of the replisome. ICLs can stall transcription. ICLs may distort the structure of chromatin and prevent the access of DNA-interacting proteins.

What is ICL repair?

In wild-type cells, interstrand crosslinks (ICLs) can be repaired by homologous recombination (HR) or non-homologous end joining (NHEJ). The Fanconi anaemia (FA) pathway, however, promotes HR-dependent stabilization of the replication fork and DNA repair.

What causes DNA crosslinks?

DNA-protein crosslinking can be caused by a variety of chemical and physical agents, including transition metals, ionizing radiation, and endogenous aldehydes, in addition to chemotherapeutic agents. Similar to DNA crosslinking, DNA-protein crosslinks are lesions in cells that are frequently damaged by UV radiation.

What is ICL in DNA?

Interstrand DNA crosslinks (ICLs) are the link between Watson-Crick strands of DNAs with the covalent bond and prevent separation of DNA strands. Since the ICL lesion affects both strands of the DNA, the ICL repair is not simple.

What is DNA repair system?

DNA repair, any of several mechanisms by which a cell maintains the integrity of its genetic code. DNA repair ensures the survival of a species by enabling parental DNA to be inherited as faithfully as possible by offspring. It also preserves the health of an individual.

What is DNA protein crosslinks?

DNA-protein crosslinks (DPCs) are formed when a nucleotide residue on DNA forms a covalent bond with a protein (or a peptide, to form a DNA-peptide crosslink, DpC). Crosslinks are particularly hazardous, as they can effectively block gene transcription and DNA replication.

What is a DNA protein crosslink?

What does Overstand mean?

(rare) To stand or insist too much or too long; overstay. verb.

What is the difference between non-homologous end joining and homology directed repair?

Non-homologous end joining (NHEJ) is a pathway that repairs double-strand breaks in DNA. NHEJ is referred to as “non-homologous” because the break ends are directly ligated without the need for a homologous template, in contrast to homology directed repair, which requires a homologous sequence to guide repair.