What is heat shock gene expression?

Abstract. Heat shock (HS) genes, or stress genes, code for a number of proteins that collectively form the most ancient and universal stress defense system. The system determines the cell capability of adaptation to various adverse factors and performs a variety of auxiliary functions in normal physiological conditions …

How does heat shock affect transcription?

Heat shock results in an altered configuration of proteins. This activates the heat-shock transcription factor (HSF), which then activates genes coding for heat-shock proteins (HSPs). HSPs have the general task of correcting the abnormal situation caused by HS-damaged proteins (see Lindquist and Craig, 1988).

Are zebrafish diploid?

As the teleost specific whole genome duplication was followed by gene (sub-) functionalisation and genome reduction, zebrafish are considered to be diploid animals, setting them apart from tetraploid cyprinids [26].

What does heat shock do to cells?

The heat shock step facilitates the entry of DNA into the bacterial cells. Recovery Broth is added to the cell suspension, and the bacteria are allowed to recover for 30 minutes at 37°C. This recovery period allows the bacteria to repair their cell walls and to express the antibiotic resistance gene.

What triggers the heat shock response?

The heat shock response is defined by the rapid expression of a class of proteins known as heat shock proteins, when a cell, tissue, or intact organism is exposed to elevated temperatures.

How many chromosomes are there in zebrafish?

25 chromosomes
The haploid zebrafish genome has 25 chromosomes, most of which are difficult to distinguish. These chromosomes contain about 1.7 X l09 base pairs of DNA, about half the mammalian genome size.

How do the zebrafish cells divide?

The organism begins as a single cell (fertilized egg) that divides successively to produce many cells, with each parent cell passing identical genetic material (two variants of each chromosome pair) to both daughter cells.

What is heat shock as applied in recombinant DNA technology?

In heat shock method, the bacterial cells are incubated with recombinant DNA on ice, followed by placing them briefly at 42o C and then putting it back on ice. The bacteria now takes up the recombinant DNA through transient pores in the bacterial cell wall.

What is plasmid insertion by heat shock treatment?

Transformation of plasmid DNA into E. coli using the heat shock method is a basic technique of molecular biology. It consists of inserting a foreign plasmid or ligation product into bacteria.

What type of protein is heat shock protein?

1 Introduction. Heat shock proteins (HSPs) are a group of proteins that function to reverse or inhibit denaturation or unfolding of cellular proteins in response to stress or high temperature. Traditionally, HSPs have also been known as molecular chaperones because of their physiological and protective roles in cells.