What is HA tag used for?

The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It is well characterized and is used extensively as a general antibody epitope tag. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.

What is HA tag in plasmid?

pSELECT HA-Tag is a family of expression plasmids designed to generate tagged proteins in mammalian cells with the human influenza hemagglutinin (HA) epitope in order to detect the tagged protein by immunocytochemistry, immunoprecipitation or Western blotting and to purify the tagged protein by affinity chromatography.

What is the HA tag sequence?

The HA tag’s nucleotide sequence is: 5′ TAC CCA TAC GAT GTT CCA GAT TAC GCT 3′ or 5′ TAT CCA TAT GAT GTT CCA GAT TAT GCT 3′ The HA tag’s amino acid sequence is: YPYDVPDYA.

How big is a HA tag?

The hemagglutinin (HA) tag is a 9-amino acid long peptide corresponding to residues 98-106 of the human influenza HA molecule, an ~63kDa surface glycoprotein required for the infectivity of the human virus.

What is a V5 tag?

V5 tag is a short peptide tag for detection and purification of proteins. The V5 tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.

What is anti HA antibody?

Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins. The product may detect cross-reacting bands in certain mammalian cells. The immunizing HA peptide (Product No.I2149) specifically inhibits the staining of the HA-tagged protein band.

What is an epitope tag?

Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The fusion gene is cloned into an appropriate expression vector for the experimental cell type and host cells are transfected.

What is an anti HA antibody?

Anti-HA is produced in rabbit using a synthetic peptide corresponding to amino acid residues of the human Influenza hemagglutinin (HA), conjugated to KLH. The antibody is affinity-purified on the immobilized immunizing peptide. Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins.

How big is the V5 tag?

V5 overview There are two sizes of the V5 tag ranging from 9–14 amino acids, although the longer tag is usually used.

How long is V5 tag?

There are two sizes of the V5 tag ranging from 9–14 amino acids, although the longer tag is usually used.

What is a Myc DDK tag?

A Myc-tag is a polypeptide protein tag derived from the c-myc gene product that can be fused to either the C- or N-terminus of a protein through recombinant DNA technology. It can facilitate the affinity purification of the tagged protein, as well as the isolation and detection of the tagged protein.

What are epitope tags?