What is Folin-Ciocalteu test?

The Folin–Ciocalteu (F-C) reaction is an antioxidant assay based on electron transfer, which measures the reductive capacity of an antioxidant. It has been widely applied in determination of the total phenol/polyphenol content of plant-derived food and biological samples.

What is Folin-Ciocalteu method used for?

The Folin-Ciocalteu (F-C) Assay was developed in 1927 for the measurement of tyrosine (1). The reagent consists of a mixture of sodium molybdate, sodium tungstate and other reagents. Upon reaction with phenols, it produces a blue color which absorbs at 765 nm.

What is the role of Folin Ciocalteu reagent in protein estimation?

Quantification and Analysis of Proteins Lowry adds phosphomolybdic/phosphotungstic acid also known as Folin-Ciocalteu reagent. This reagent interacts with the cuprous ions and the side chains of tyrosine, tryptophan, and cysteine to produce a blue-green color that can be detected between 650 nm and 750 nm.

Why sodium carbonate is used in Folin-Ciocalteu method?

In Folin ciocalteu method sodium carbonate plays a major role as its presence there helps in getting high yield of phenolate ions.

Why Sodium carbonate is used in Folin-Ciocalteu method?

What is the colour of Folin Ciocalteu reagent?

The Folin-Ciocalteu reagent * yields a blue colour with phenolic compounds, cysteine2, and some buffers and tuonosaccllnricles when prcscnt in high conccn- Iration”*“.

Which method is used for protein estimation?

The Lowry method has been widely used for protein determination for many decades, due to its simplicity and availability. However, besides aromatic amino acids, a wide range of other compounds react with the Folin–Ciocalteu reagent [30].

Why Lowry method is used for protein estimation?

The principle behind the Lowry method of determining protein concentrations lies in the reactivity of the peptide nitrogen[s] with the copper [II] ions under alkaline conditions and the subsequent reduction of the Folin- Ciocalteay phosphomolybdic phosphotungstic acid to heteropolymolybdenum blue by the copper- …

How do you use Folin Ciocalteu reagent?

To perform an assay add x cm3 of sample (where x < 0.6) containing 5–100 μg of protein as required to (0.6– x) cm3 of water. Then add 3 cm3 of the alkaline copper solution. The solutions must then be mixed well and allowed to stand for 10 min at room temperature.

What is the purpose of Lithium Li ions in the Folin Ciocalteu reagent?

The FCR (Folin and Ciocalteu, 1927) provides an improvement over the Folin–Denis reagent with the addition of lithium sulfate to the reagent. The lithium salts reduce the amount of precipitate that can form when high concentrations of reagent are used to increase the assay’s reactivity.