What is Folin-Ciocalteu phenol reagent?

The Folin–Ciocâlteu reagent (FCR) or Folin’s phenol reagent or Folin–Denis reagent, also called the gallic acid equivalence method (GAE), is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants.

How do you make Folin-Ciocalteu reagent?

Dissolve 10 g of sodium tungstate and 2.5 g of sodium molybdate in 70 ml of water. Add 5 ml of 85% phosphoric acid and 10 ml of concentrated hydrochloric acid. Reflux for 10 hours. Add 15 g of lithium sulfate, 5 ml of water, and 1 drop of bromine.

What is Folin-Ciocalteu reagent made of?

The Folin-Ciocalteu (F-C) Assay was developed in 1927 for the measurement of tyrosine (1). The reagent consists of a mixture of sodium molybdate, sodium tungstate and other reagents. Upon reaction with phenols, it produces a blue color which absorbs at 765 nm.

How do you spell Kjeldahl?

“Kjeldahl.” Merriam-Webster.com Medical Dictionary, Merriam-Webster, https://www.merriam-webster.com/medical/Kjeldahl.

What is Folin reagent used for?

Folin’s reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used as a derivatizing agent to measure levels of amines and amino acids. The reagent reacts with them in alkaline solution to produce a fluorescent material that can be easily detected.

How do I prepare 10 Folin-Ciocalteu?

Add 50 mL phosphoric acid 85% (ρ20 = 1.71 g/mL), and 100 mL of concentrated hydrochloric acid (ρ20 = 1.19 g/mL). Bring to the boil and reflux for 10 hours. Then add 150 g of lithium sulfate, Li2SO4. H2O, and a few drops of bromine and boil for 15 minutes.

What is the Folin Lowry method?

A sensitive method of protein assay in which protein reacts both with an alkaline copper tartrate solution and with the Folin‐Ciocalteu reagent to give a blue colour, the absorbance of which is measured at 750 nm.