What is excitation wavelength fluorescence?

A fluorescence excitation spectrum is when the emission wavelength is fixed and the excitation monochromator wavelength is scanned. In this way, the spectrum gives information about the wavelengths at which a sample will absorb so as to emit at the single emission wavelength chosen for observation.

What color is hex fluorophore?

Four differently colored fluorophores (FAM: green; HEX: orange; ROX: red; and Cy5: blue) and one universal quencher (DABCYL: black) are used to depict the principal of MCPC.

Why do fluorophores fluoresce?

Fluorophores are molecules that, upon absorbing light energy, can reach an excited state, then emit light energy. The three-stage process of excitation, excited lifetime, and emission is called fluorescence. Fluorophores absorb a range of wavelengths of light energy, and also emit a range of wavelengths.

What color is fluorescent light?

Like sunlight, fluorescent light is also white light, and it is also made up of many different wavelengths.

What color temperature is fluorescent?

2700K – 6500K
Fluorescent (2700K – 6500K) They have a very soft light that is more efficient that an incandescent bulb, and can offer an output similar to HMI lights. Depending on the mix of phosphors in the bulbs, the color temperature can range from tungsten up to natural daylight.

How do you choose excitation wavelength for fluorescence?

In order to achieve maximum fluorescence intensity, the fluorochrome is usually excited at the wavelength at the peak of the excitation curve. At this point the emission detection is selected at the peak wavelength of the emission curve.

What color is Cy5?

Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

What color is TRITC?

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

What is the difference between Fluorochrome and fluorophore?

Yes, fluorophore and fluorochrome refer to the same thing. Fluorophores, or fluorochromes, are fluorescent chemical compounds that are capable of absorbing light from a laser and re-emitting the light within a range of wavelengths upon excitation.