What is epitope prediction?
What is epitope prediction?
One of the most important applications of immunoinformatics is the prediction of a variety of specific epitopes for B cell recognition and T cell through MHC class I and II molecules. This method reduces costs and time compared to laboratory tests.
How do you predict T cell epitopes?
User can predict the T cell immunogenicity using 7-allele method (Paul et. al. 2015), immunogenicity method and combined method (IEDB recommended). The combined method predicts the final score that combines the predictions from 7-allele method and immunogenicity method.
What is epitope and Paratope?
An epitope, also known as antigenic determinant, is the part of an antigen that is recognized by the immune system, specifically by antibodies, B cells, or T cells. The epitope is the specific piece of the antigen to which an antibody binds. The part of an antibody that binds to the epitope is called a paratope.
How does epitope mapping work?
The molecular biological technique of site-directed mutagenesis (SDM) can be used to enable epitope mapping. In SDM, systematic mutations of amino acids are introduced into the sequence of the target protein. Binding of an antibody to each mutated protein is tested to identify the amino acids that comprise the epitope.
What epitope means?
An epitope or antigenic determinant is a group of amino acids or other chemical groups exposed on the surface of a molecule, frequently a protein, which can generate an antigenic response and bind antibody.
Why is epitope identification important?
Epitope identification using high-performance immuno-analytic tools can be very useful in various applications in the field of epitope mapping, including the design of peptide-based vaccines, identification of immunological processes, prediction of epitopes used in the diagnosis of diseases, determination of features …
How do you identify an epitope?
B-cell epitopes can be identified by different methods including solving the 3D structure of antigen-antibody complexes, peptide library screening of antibody binding or performing functional assays in which the antigen is mutated and the interaction antibody-antigen is evaluated [3, 4].
What are the types of B cell epitope prediction?
B-cell epitopes are usually composed of two types, linear or conformational B-cell epitopes. Linear B-cell epitopes are continuous amino acid sequence fragments. Conformational B-cell epitopes consist of the amino acid that is mainly made up of different residues formed by protein folding.
What are the types of epitopes?
Two types of epitopes i. continuous and ii. discontinuous epitopes participate in epitope-antibody-reactivities (EAR). B cell epitopes are most commonly discontinuous (also called conformational or assembled), consisting of segments of multiple chains brought together by the folding of the protein (antigen) [10].
What is the epitope?
What is the function of an epitope?
An epitope is the part of an antigen that the host’s immune system recognizes, eliciting the immune response to an invading pathogen. It specifically binds to the corresponding antigen receptor on the immune cell (such as a B cell) and binding only occurs if the structures are complementary.
What are B cell epitopes?
B-cell epitopes can be defined as a surface accessible clusters of amino acids, which are recognized by secreted antibodies or B-cell receptors and are able to elicit cellular or humoral immune response [1].
