What is ecfp?

ECFP is a basic (constitutively fluorescent) cyan fluorescent protein published in 1996, derived from Aequorea victoria. It has low acid sensitivity.

How many base pairs are in EGFP?

Most commercially available genes for GFP and similar fluorescent proteins are around 730 base-pairs long.

What is Ecfp fingerprint?

(5)Extended-connectivity fingerprints (ECFPs) are a recently developed fingerprint methodology explicitly designed to capture molecular features relevant to molecular activity. While not designed for substructure searching, they are well suited to tasks related to predicting and gaining insight into drug activity.

What are Maccs keys?

MACCS keys are 166-bit 2D structure fingerprints that are commonly used for the measure of molecular similarity. Because each bit is either on (i.e., 1) or off (i.e., 0), MACCS 166 keys can represent more than 9.3 \times 10^{49} distinct fingerprint vectors.

What is Morgan algorithm?

The core idea behind ECFPs traces its origin to the Morgan algorithm. This algorithm seeks to assign a unique, sequential atom numbering for any given molecule. Unique atom numbering underpins all widely-used methods for molecule identification, including those used by InChI and the CAS Registry.

What is rotatable bond count?

The number of rotatable bonds (RBN) is the number of bonds which allow free rotation around themselves. These are defined as any single bond, not in a ring, bound to a nonterminal heavy atom. Excluded from the count are amide C–N bonds because of their high rotational energy barrier (F. Veber et al., J. Med.

What is molecular fingerprint?

The molecular fingerprint is a way to describe a molecular structure that can convert a molecular structure into a bit string [11, 12]. Since molecular fingerprint encodes the structure of a molecule, it is a useful method to describe the structural similarity among the molecules as a molecular descriptor.

How large is GFP in kDa?

27 kDa
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.

What are the different types of mutations in ECFP?

ECFP was derived from W7with the following mutations: M1_S2insV/F64L/S65T/K212N/H231LM1_S2insV/F64L/S65T/K212N/H231L Note: The ECFP line is sometimes incorrectly reported as having the mutation N149I (instead of N146I) with respect to wild-type avGFP (e.g. see Supp Table 2 of Shaner et al 2005… since corrected in the online media).

How to obtain enhanced green fluorescent protein (EGF) sequence with restriction sites?

Enhanced green fluorescent protein. To obtain this DNA and protein sequence with restriction sites, please download SnapGene or the free SnapGene Viewer. Your time is valuable! × Please choose an application for opening sequence files. Don’t have either application? Download SnapGene or SnapGene Viewer.

What is the pKa maturation (Min) of EGFP cells?

EC(M-1cm-1) QY Brightness pKa Maturation (min) Lifetime (ns) 488 507 55,900 0.6 33.54 6.0 25.0 2.6 Edit States/AttributesEdit States/Attributes EGFP OSER Measurements % Normal Cells OSER/NE ratio Cell Type Reference 76.5 ± 6.9 (10000 cells) HeLa Cranfill et al. (2016) 76.5 ± 6.9 (10000 cells) HeLa

What does ECFP stand for?

ECFP is a basic (constitutively fluorescent) cyan fluorescent protein published in 1996, derived from Aequorea victoria. It has low acid sensitivity.